Potential Nitrogen Fixing Rhizobia Isolated from Some Wild Legumes of Nagaland Based on RAPD with Nif-directed Primer and Their Biochemical Activities

Maman Megu; A. Paul; Chitta Ranjan Deb

doi:10.18006/2024.12(4).588.605

Authors

Maman Megu Department of Botany, Nagaland University, Lumami 798627, Nagaland, India https://orcid.org/0000-0003-1848-195X
A. Paul Department of Botany, Nagaland University, Lumami 798627, Nagaland, India
Chitta Ranjan Deb Department of Botany, Nagaland University, Lumami 798627, Nagaland, India https://orcid.org/0000-0002-1361-1025

DOI:

https://doi.org/10.18006/2024.12(4).588.605

Keywords:

Wild legumes, Nodules, Rhizobia, RNB, RAPD, Symbiotic association

Abstract

Wild legumes are widely dispersed and can survive in challenging environments as bacteria dwell in their nodules and help each other. Although Nagaland is home to many wild legume varieties, research on the microbial diversity that goes along with them is still in its infancy. This work aimed to characterize several wild legume root nodules and distinguish possible rhizobial isolates using RAPD and nif-directed RPO1 primer. Nodule bacteria were isolated in Yeast extract culture media. Based on their colony morphology, 150 isolates were selected for performing RAPD with nif-directed RPO1 primer. Eighty-four isolates were bonded with RPO1 primer, and a few biochemical tests were conducted on RPO1-positive isolates. Activities that promoted plant development were also investigated for these isolates. Of all the isolates, 18 exhibited phosphate solubilization capacity, while 38 isolates were found to produce IAA. This study entails a large variety of rhizobia in the nodules, which were able to promote growth. Hence, these isolates promise to be bio-fertilizers that could improve agricultural operations.

Author Biographies

Maman Megu, Department of Botany, Nagaland University, Lumami 798627, Nagaland, India

Department of Botany, Nagaland University, Lumami 798627, Nagaland, India

A. Paul, Department of Botany, Nagaland University, Lumami 798627, Nagaland, India

Department of Botany, Nagaland University, Lumami 798627, Nagaland, India

Chitta Ranjan Deb, Department of Botany, Nagaland University, Lumami 798627, Nagaland, India

Department of Botany, Nagaland University, Lumami 798627, Nagaland, India

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