Antibacterial Efficacy of Zinc oxide nanoparticles against Serratia marcescens  (ATCC 43862) and Enterococcus faecalis (ATCC 29121)

Lee Jun Jie; Loh Zhe Chi; Ling Shing Wong; Ranjithkumar Rajamani; Sinouvassane Djearamane

doi:10.18006/2022.10(5).1069.1075

Authors

Lee Jun Jie Department of Biomedical Science, Universiti Tunku Abdul Rahman (UTAR), Kampar, 31900, Perak, Malaysia
Loh Zhe Chi Department of Biomedical Science, Universiti Tunku Abdul Rahman (UTAR), Kampar, 31900, Perak, Malaysia
Ling Shing Wong Life Science Division, Faculty of Health and Life Sciences, INTI International University, Nilai, 71800, Malaysia https://orcid.org/0000-0002-5869-0804
Ranjithkumar Rajamani Viyen Biotech LLP, Coimbatore, Tamil Nadu - 641 031, India https://orcid.org/0000-0001-7438-0200
Sinouvassane Djearamane Department of Biomedical Science, Universiti Tunku Abdul Rahman (UTAR), Kampar, 31900, Perak, Malaysia https://orcid.org/0000-0002-8251-662X

DOI:

https://doi.org/10.18006/2022.10(5).1069.1075

Keywords:

Anti-bacterial, Zinc oxide nanoparticles, Serratia marcescens, Enterococcus faecalis, Minimum inhibitory concentration

Abstract

Zinc oxide nanoparticles (ZnO NPs) are a novel and alternative biomaterial for active biomedical applications among all metal and metallic oxide nanoparticles due to less toxicity and biocompatibility with human cells. In this study, we studied the growth curve of Serratia marcescens and Enterococcus faecalis to identify the mid-log phase of the bacterial growth to perform the exposure with ZnO NPs for investigating the antibacterial efficacy. The INT assay was used to determine the anti-bactericidal efficiency of ZnO NPs against S. marcescens and E. faecalis. The results showed that both the test bacteria attained the mid-log phase at the 5^th hour. The determination of minimum inhibitory concentration (MIC) demonstrated a higher efficacy of ZnO NPs on the Gram-positive bacterium E. faecalis compared to the Gram-negative bacterium S. marcescens. The present study reports a higher susceptibility of Gram-positive bacterium over Gram-negative bacterium to the treatment of ZnO NPs.

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