Optimization of Total Flavonoid Extraction From the Helicteres hirsuta Lour. Roots by Bath Ultrasound Assisted method and cytotoxic activities of these Flavonoids

Tong Thi Hue; Dang Thanh Long; Van Thi My Le; Hoang Thi Ngoc Han; Nguyen Thi Quynh Trang

doi:10.18006/2023.11(1).179.189

Authors

Tong Thi Hue Institute of Biotechnology, Hue University, PhuThuong, Hue, ThuaThien Hue, Vietnam https://orcid.org/0009-0003-8421-7433
Dang Thanh Long Institute of Biotechnology, Hue University, PhuThuong, Hue, ThuaThien Hue, Vietnam https://orcid.org/0000-0001-9519-1118
Van Thi My Le University of Sciences, Hue University, 77 Nguyen Hue, Thua Thien Hue, Vietnam https://orcid.org/0009-0002-5416-0635
Hoang Thi Ngoc Han Institute of Biotechnology, Hue University, PhuThuong, Hue, ThuaThien Hue, Vietnam https://orcid.org/0009-0004-7767-9418
Nguyen Thi Quynh Trang University of Education, Hue University, 32 Le Loi, Thua Thien Hue, Vietnam https://orcid.org/0000-0002-1206-4680

DOI:

https://doi.org/10.18006/2023.11(1).179.189

Keywords:

Flavonoid, Helicteres hirsuta Lour., Extract, Root, RMS, LC-MS/MS, Human leukemia cell line

Abstract

This study was carried out to optimize the various approaches to analyze the effects of various variables on the total flavonoid content extraction from the roots of Helicteres hirsuta L. The existence of various compounds in the methanol fraction was accessed by using LC-MS/MS analysis. The results of the study identified the ideal parameters such as times (30 minutes); methanol solvent concentration (50%); ultrasonic frequency (12 Hz); and material/solvent ratio [1:30 (w/v)] for extracting the highest total flavonoids from the roots of H. Hirsuta. The study's results suggested that the total flavonoid value was 3.52684 (mg Catechin/g extract). The verified experiment obtained an actual value of 5.205 (mg Catechin/g extract). Further, the results of the study suggested the presence of 20 compounds of a flavonoid nature (66.667%) appearing in the purified methanol fractional extract. These compounds can inhibit DPPH free radicals at 50%, with an IC₅₀ value of 536.760 g/mL, and they also have inhibitory activity on the growth of cancer cell lines with IC₅₀ values ranging from 115.81 and 219.17g/mL. The human leukemia cell line (HL-60) exhibits the most significant cytotoxic response to a methanol extract from H. hirsuta root with an IC₅₀ value of 115.81 g/mL.

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