Synergistic Effect of Plant Growth Regulators on Micropropagation of Eclipta alba:  A Plant with Diverse Medicinal Properties

Vasudha Datta; Lalit Sharma; Diwakar Aggarawal; Anil K Sharma; Kuldeep Dhama

doi:10.18006/2022.10(6).1432.1440

Authors

Vasudha Datta Department of Biotechnology, Maharishi Markandeshwar Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana-(Ambala) 133207, Haryana, India. https://orcid.org/0000-0002-0246-2968
Lalit Sharma Department of Biotechnology, Maharishi Markandeshwar Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana-(Ambala) 133207, Haryana, India. https://orcid.org/0000-0003-0415-9767
Diwakar Aggarawal Department of Biotechnology, Maharishi Markandeshwar Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana-(Ambala) 133207, Haryana, India. https://orcid.org/0000-0001-6920-4341
Anil K Sharma Department of Biotechnology, Maharishi Markandeshwar Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana-(Ambala) 133207, Haryana, India. https://orcid.org/0000-0002-9768-1644
Kuldeep Dhama Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, UP, India. https://orcid.org/0000-0001-7469-4752

DOI:

https://doi.org/10.18006/2022.10(6).1432.1440

Keywords:

Auxins, Medium strength, Plant growth regulators, RAPD, Shoot multiplication, Tissue culture

Abstract

Synergism between various plant growth regulators is reported to be a key factor for the development of efficient in vitro propagation for any plant. Therefore, the current study examines the synergistic impact of plant growth regulators on in vitro propagation of Eclipta alba, an important medicinal plant possessing diverse medicinal properties. For the establishment of aseptic cultures, nodal segments were employed as explants on MS medium supplemented with 2.5 μM of 6-benzyle adenine (BA). Varying concentrations of BA and Kinetin (KIN)(0.0-5.0 μM), either alone or in combination with α- naphthalene acetic acid (NAA @ 0.0-5.0 μM) and indole 3-acetic acid (IAA@ 0.0-5.0 μM), were found to be effective for promoting shoot proliferation. Compared to KIN, BA was found to promote shoot proliferation and elongation more effectively. Further, the addition of 0.5μM NAA in the MS medium supplemented with 2.5 μM of BA increased shoot multiplication and elongation frequency from 58 and 17 percent to 65 and 21 percent respectively. The rooting frequency was found to be maximum on 1/2 strength MS medium supplemented with 5.0 μM of indole 3-butyric acid (IBA), which was found to be a superior auxin for inducing roots as compared to the NAA and IAA. With a 75% survival rate, in vitro raised plantlets were effectively acclimatized first in a poly house and later under greenhouse conditions. Molecular analysis was carried out using RAPD markers, with results indicating that the micropropagated plants were genetically identical to the mother plant. The developed micropropagation protocol for E. alba can be used at the commercial level for the mass multiplication of plants.

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