Molecular identification of scale insect (Eulecanium giganteum) in Hibiscus rosa-sinensis

Suganthi M; Logeshwaran R; Abirami G; Rupa Shree B; Anandaraj P; Senthilkumar P

doi:10.18006/2022.10(4).797.804

Authors

Suganthi M Department of Biotechnology, Vels Institute of Science, Technology & Advanced Studies (VISTAS), Pallavaram 600117, TamilNadu, India https://orcid.org/0000-0003-4433-6887
Logeshwaran R Department of Biotechnology, Vels Institute of Science, Technology & Advanced Studies (VISTAS), Pallavaram 600117, TamilNadu, India
Abirami G Department of Biotechnology, Vels Institute of Science, Technology & Advanced Studies (VISTAS), Pallavaram 600117, TamilNadu, India
Rupa Shree B Department of Biotechnology, Vels Institute of Science, Technology & Advanced Studies (VISTAS), Pallavaram 600117, TamilNadu, India
Anandaraj P Department of Biotechnology, Vels Institute of Science, Technology & Advanced Studies (VISTAS), Pallavaram 600117, TamilNadu, India
Senthilkumar P Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur 603203, Chennai, TamilNadu, India https://orcid.org/0000-0003-0987-4946

DOI:

https://doi.org/10.18006/2022.10(4).797.804

Keywords:

Insect DNA, MtCOI, PCR, Pest management, Sap-sucking pest, Sequencing

Abstract

Hibiscus rosa-sinensis is a widely grown evergreen valuable medicinal, ornamental species planted in India. Scale insects are small herbivorous insects found on all continents and they are serious sap sucking pests of many ornamental plants. These scale insects are undetectable due to their tiny size, basic morphology, and polyphagous feeding nature. Hence, the management of these tiny insects become a serious concern across the globe. To afford a prospective solution to the problem, an accurate, simple, and developmental-stage-independent identification method is required, hence this study attempted the molecular identification of scale insect in Hibiscus rosa-sinensis using mitochondrial gene Cytochrome Oxidase Subunit I (mtCOI) sequencing. The experiment was carried out by isolating insect DNA using a modified CTAB method. Through two or three rounds of error-prone PCR followed by a steady procedure to amplify a mtCOI region. This region of mtCOI has been used as a standard DNA barcode for a diverse array of taxa. The confirmation has been done by sequencing of mtCOI which suggest the highest similarities with Eulecanium giganteum. This study addresses the questions of biodiversity and molecular characterization of scale insects. Further, the information obtained in this study provides baseline data for future crop improvement programs and integrated pest management strategies.

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