Biogenic Synthesis and Characterization of Silver Nanoparticles (AgNPs) Produced by Indigenous Microorganisms Isolated from Banana (Musa spp) Soils

Michelle Ann M. Calubaquib; Evelyn F. Delfin; Florinia E. Merca; Lucille C. Villegas; Andre Freire Cruz; Erlinda S. Paterno

doi:10.18006/2023.11(1).105.118

Authors

Michelle Ann M. Calubaquib Isabela State University Cabagan Campus – Isabela – Philippines https://orcid.org/0000-0002-9332-8421
Evelyn F. Delfin University of the Philippines Los Banos – Laguna – Philippines https://orcid.org/0000-0002-3980-9822
Florinia E. Merca University of the Philippines Los Banos – Laguna – Philippines https://orcid.org/0000-0001-7650-5433
Lucille C. Villegas University of the Philippines Los Banos – Laguna – Philippines https://orcid.org/0000-0002-3450-7841
Andre Freire Cruz Kyoto Prefectural University – Kyoto- Japan https://orcid.org/0000-0001-9349-2742
Erlinda S. Paterno University of the Philippines Los Banos – Laguna – Philippines https://orcid.org/0000-0002-6979-8853

DOI:

https://doi.org/10.18006/2023.11(1).105.118

Keywords:

Aspergillus , Bacillus , Microorganism, Nanotechnology, Penicillium

Abstract

This research focused on the screening of indigenous microorganisms isolated from banana soils for their capability to synthesize silver nanoparticles (AgNPs) extracellularly. Ninety-five isolates were screened for AgNP production. The cell-free extracts of these isolates were added to silver nitrate (AgNO₃) aqueous solution and were observed for color changes from original pale yellow to dark brown. Ten isolates (3 bacteria and 7 fungi) were found capable of producing AgNPs. Bacterial isolates B2, B3, and B5 were molecularly identified as Bacillus aryabhattai, Priestia megaterium, and B. megaterium, respectively. The AgNPs produced by these bacterial isolates were circular and showed an absorbance peak at approximately 420 nm. On the other hand, the fungal isolates F2, F3, and F43 were molecularly identified as Penicilliumcitrinum, P. glaucoroseum, and P. oxalicum. The AgNPs produced by the Penicillium spp were aggregated, circular and showed absorbance peaks at 420 nm. The other four fungal isolates, F7, F24, F29, and F40, were identified as Aspergillus flavus, A. terreus, and A. japonicum (F29 and F40), respectively. The AgNPs produced by the Aspergillus spp. were circular and showed absorbance peaks between 420 nm and 450 nm. The continuous search for novel isolates that can carry out the biogenic synthesis of AgNPs remains the focus of nanotechnological research. This study confirms microorganisms of Bacillus, Penicillium, and Aspergillus genera can effectively biosynthesize AgNPs.

Author Biography

Michelle Ann M. Calubaquib, Isabela State University Cabagan Campus – Isabela – Philippines

University of the Philippines Los Banos – Laguna – Philippines

Isabela State University Cabagan Campus – Isabela – Philippines

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