SEROTYPES, TOXINS AND ANTIBIOTIC RESISTANCE OF Escherichia coli (E.COLI) STRAINS ISOLATED FROM DIARRHEIC RABBITS IN PHU VANG,  THUA THIEN HUE

Huynh Van  Chuong; Le Thi Lan  Phuong; Dang Thanh  Long

doi:10.18006/2021.9(5).610.617

Authors

Huynh Van Chuong Institute of Biotechnology, Hue University, provincial road No. 10, Phu Vang, Thua Thien Hue, Vietnam
Le Thi Lan Phuong University of Agriculture and Forestry, Hue University, Vietnam
Dang Thanh Long Institute of Biotechnology, Hue University, provincial road No. 10, Phu Vang, Thua Thien Hue, Vietnam

DOI:

https://doi.org/10.18006/2021.9(5).610.617

Keywords:

Antibiotic susceptibility, Diarrhea, E. coli, Resistant, Rabbits

Abstract

This study was conducted to determine the prevalence of E. coli in rabbits, their biochemical and serological characteristics, common virulence genes, and antibiotic resistance. The diarrhea rabbit feces were collected from households and rabbit farms in Phu Vang - Thua Thien Hue with a total of 250 samples for testing. The results showed that rabbits age from 31 to 45 days old had the highest incidence of diarrhea disease caused by E.coli (92.0%) and the lowest infection rate was observed in rabbits over 60 days old with an infection rate of 30%. Further, the isolated E.coli strains tested biochemical characteristics showed 100% motile, positive for indole and methyl red, fermenting glucose and lactose. Simultaneously these strains were detected belong to 7 serotypes O103, O157, O158, O169, O44, O125, O153 and susceptible to cefuroxime (95.45%), akamicin (86.37%), streptomycin (81.82%), amoxicillin (81.82%), tetracycline (68.18%), colistin (68.18%), ampicillin (63.63%), gentamycin (59.10%) and levofloxacin (50.0%), whilst resistant to doxycycline (100%), sulfamethoxazole-bactrim (95.46%), and neomycin (86.37%). By using PCR assay for detection of virulence genes of the isolated E. coli strains, there were 7 strains carried virulence genes, of which 4/7 E. coli strains carried eaeA and tsh genes (57.14%), 2/7 strains carried stx2 gene (28.57%); 1/7 E. coli strains carried stx1 gene (14.28%) and the F4, F5 and F6 genes were not found in all serotypes in this study.

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