Effect of the combined application of Lampung Robusta Coffee Extract and  Lactobacillus acidophilus on the Ileum and Caecum Histopathology in  Salmonella enterica infected Balb/C Mice

Indah Amalia  Amri; Dhyas  Meilani; Muhammad Zuliono  D. R. P.; Dahliatul  Qosimah; Dodik  Prasetyo; Djalal  Rosyidi; Lilik Eka  Radiati

doi:10.18006/2022.10(3).660.667

Authors

Indah Amalia Amri Faculty of Veterinary Medicine, Brawijaya University, Indonesia
Dhyas Meilani Faculty of Veterinary Medicine, Brawijaya University, Indonesia
Muhammad Zuliono D. R. P. Faculty of Veterinary Medicine, Brawijaya University, Indonesia
Dahliatul Qosimah Faculty of Veterinary Medicine, Brawijaya University, Indonesia
Dodik Prasetyo Faculty of Veterinary Medicine, Brawijaya University, Indonesia
Djalal Rosyidi Faculty of Animal Science, Brawijaya University, Indonesia
Lilik Eka Radiati Faculty of Animal Science, Brawijaya University, Indonesia

DOI:

https://doi.org/10.18006/2022.10(3).660.667

Keywords:

Robusta Lampung Coffea, Lactobacillus acidophilus, Salmonella enteric, Ileum, Caecum, Histopathology

Abstract

Salmonella enterica is a gram-negative bacterium that can cause Salmonellosis and gastroenteritis in humans and animals. Further, this bacterial infection is also associated with the reactive oxygen species (ROS) by lipid peroxidase that can destroy the intestinal cell's membrane. This study aimed to evaluate the preventive effect of the combined application of Lampung Robusta coffee extract and Lactobacillus acidophilus on the Ileum and Caecum Histopathology in Salmonella enterica infected Mice. In this study, male Balb-c mice aged between 8-10 weeks and weight 20-25 grams were used, these experimental animals were divided into six experimental groups namely K^-(Negative control without any infection), K⁺(Positive control with S. enterica), KL (Only L. acidophilus treated mice), P1, P2, and P3 were given a preventive extract of coffee with a concentration of 250 mg/kg BW, 500 mg/kg BW, and 750 mg/kg BW respectively and L. acidophilus to S.enterica infected mice and arrange in completely Randomized Design. Descriptive histopathological analyses were carried out after HE staining and villi's length and width for ileum's histopathology and counting goblet cells for caecum's histopathology was scored. The results of the study revealed that administration of Robusta Coffee extract @ 250 mg/ kg BW and L. acidophilus has a preventive effect on the ileum and caecum damage caused by salmonellosis.

References

Adenin, I. (2019). The Role of Inflammatory Components Due to Insertion of Contraceptive Devices in the Womb and Relationship with Increased Levels of Glycodelin A. Ejki, 2, 156-161.

Anggarini, W. (2011). Effect of Indigenous Probiotic-Based Combination Yogurt on Histological Profile and Content of Immunoglobulin A (Ig A) Intestine Smooth Intestinal Rats. [Unpublished PhD thesis], Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Indonesia.

Arguello, H., Estellé, J., Zaldívar-López, S., Jiménez-Marín, A., et al. (2017). Early Salmonella typhimurium Infection in Pigs Disrupts Microbiome Composition and Functionality Principally at The Ileum Mucosa. Scientific Reports, 8, 7788. https://doi.org/10.1038/s41598-018-26083-3. DOI: https://doi.org/10.1038/s41598-018-26083-3

Asminarti (2014). Effect of Various Concentrations of Leaf and Peel Extract of Mangosteen (Garcinia mangostana L.) on In Vitro Growth Inhibitory Zones of Salmonella enteritidis Bacteria. [Unpublished PhD thesis]. Fakultas Keguruan dan Ilmu Pendidikan Universitas Muhammadiyah Malang, Indonesia

Balqis, U., Hanafiah, M., Januari, C., Salim, M. N., Aisyah dan S., & Fahrimal, Y. (2015). The number of goblet cells in the small intestine of native chickens (Gallus domesticus) naturally infected with Ascaridia galli. Jurnal Medika Veterinaria, 9 (1), 64. DOI: https://doi.org/10.21157/j.med.vet..v9i1.3001

Bergstrom, K.S.B., Guttman, J.A., Rumi, M., Ma, C., et al. (2008). Modulation of Intestinal Goblet Cell Function during Infection by an Attaching and Effacing Bacterial Pathogen. Infection and Immunity, 7, 796–811. DOI: https://doi.org/10.1128/IAI.00093-07

Erben, U., Loddenkemper, C., Doerfel, K., Spieckermann, S., et al. (2014). A guide to histomorphological evaluation of intestinal inflamation in mouse model. International Journal of Clinical and Experimental Pathology , 7(8),4557-4576.

Galdeano, C.M., Cazrola, S.I., Dumit, J.M.L., Vélez, E., Perdigón, G. (2019). Beneficial Effects of Probiotic Consumption on the Immune System. Annals of Nutrition & Metabolism, 74, 115–124. DOI: https://doi.org/10.1159/000496426

Grace, H. A. (2017). Organoleptic Inventory, Caffeine Content, and Chlorogenic Acid in Robusta Coffee (Coffea Canephora L.) in Tanggamus Regency. [Unpublished PhD thesis], Fakultas Pertanian, Universitas Lampung, Indonesia.

Harimurti, S., & dan Rahayu, E. S. (2009). Intestinal Morphology of Broiler Chickens Supplemented With Single Strain And Mixed Probiotics. Agritech, 29(3).

Juliantari, D.K.K. (2018). Characteristics of Robusta (Coffea canephora) Coffee Dregs Extract In The Treatment Of Ethanol Solvent Concentration And Maceration Temperature. Jurnal Rekayasa dan Manajemen Agroindustri, 6(3), 243-249. DOI: https://doi.org/10.24843/JRMA.2018.v06.i03.p08

Keraru, E. N. (2017). Effect of Variation in Dose of Robusta Coffee (Coffea canephora) Manggarai infusion on the Laxative Effect on Female White Rats. [Unpublished PhD thesis], Fakultas Keguruan dan Ilmu Pendidikan, Universitas Sanata Dharma, Indonesia.

Liu, X., Xia, B., He, T., Dan Li, D. (2019). Oral Administration of a Select Mixture of Lactobacillus and Bacillus Alleviates Inflammation and Maintains Mucosal Barrier Integrity in the Ileum of Pigs Challenged with Salmonella Infantis. Microorganisms, 7(5),135. DOI: https://doi.org/10.3390/microorganisms7050135

Mansson, L. E., Montero, M., Zarepour, M., Bergstrom, K.S., et al. (2012). MyD88 Signaling Promotes Both Mucosal Homeostatic and Fibrotic Responses during Salmonella induced colitis. American Journal of Physiology-Gastrointestinal and Liver Physiolog, 303, G311-G323. DOI: https://doi.org/10.1152/ajpgi.00038.2012

Marchelletta, R. R., Gareau, M.G., Okamoto, S., Guiney, D.G., Barrett, K.E., & Fierer, J. (2014). Salmonella-induced Diarrhea Occurs in the Absence of IL-8 Receptor (CXCR2)-Dependent Neutrophilic Inflammation. Journal of Infectious Diseases, 212(1), 128–136. DOI: https://doi.org/10.1093/infdis/jiu829

Muhammad A. Y., & dan Mumun, N. (2015). Effect of Robusta Coffee Extract (Coffea robusta) as a Growth Inhibitor of Staphylococcus aureus. Seminar Nasional XII Pendidikan Biologi FKIP UNS.

Putra, A. L. O., dan Kusdiyantini, E. (2018). Isolation and Identification of Lactic Acid Bacteria from Fish-Based Fermented Food (Inasua) which is traded in Maluku-Indonesia. Jurnal Biologi Tropika 1 (2), 6-12. DOI: https://doi.org/10.14710/jbt.1.2.6-12

Rizkiani, I. (2009). Effect of Giving Oral Doses of Coffee for 30 Days on Gastric Histology of Wistar Rats. [Unpublished PhD thesis], Fakultas Kedokteran, Universitas Semarang, Indonesia.

Rosales, C. (2018). Neutrophil: A Cell with Many Roles in Inflammation or Several Cell Types?. Frontiers in Physiology, 9, 113. DOI: https://doi.org/10.3389/fphys.2018.00113

Santos, R. I., Tsolis, R.M., Bäumler, A.J., & Adams, L.G. (2003). Pathogenesis of Salmonella-induced enteritis. Brazilian Journal of Medical and Biological Research, 36, 3-12. DOI: https://doi.org/10.1590/S0100-879X2003000100002

Selviana, B. Y. (2015). Efek of Coffee and Stress With the Incidence Of Gastritis. Jurnal Majorit, 4 (2). Retrived from file:///C:/Users/user/Downloads/518-1015-2-PB.pdf.

Senditya DKK (2011). Effects of Probiotics and Simplicia Combination of Black Cincau Leaves (Mesona palustris BL) In

Vivo: Literature Review. Jurnal Pangan dan Agroindustri, 2(3): 141-151.

Songhet, P., Barthel, M., Stecher, B., Müller, A.J. et al. (2011). Stromal IFN-γR-signaling modulates goblet cell function during Salmonella Typhimurium infection. PLoS ONE, 6(7), e22459. doi: 10.1371/journal.pone.0022459. DOI: https://doi.org/10.1371/journal.pone.0022459

Stecher, B., Macpherson, A.J., Hapfelmeier, S., Kremer, M., Stallmach, T., Hardt, W.D. (2005). Comparison of Salmonella enterica Serovar Typhimurium Colitis in Germfree Mice and Mice Pretreated with Streptomycin. Infection and Immunity, 73(6), 3228–3241. DOI: https://doi.org/10.1128/IAI.73.6.3228-3241.2005

Wulandari, E. (2016). Effects of Rambutan Fruit Peel Extract on MDA and SOD Levels of Rats Exposed to Cigarette Smoke. [Unpublished PhD thesis], Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Semarang, Indonesia.