Evaluation of DNA Isolation and Amplification from Various Organs Preserved through Frozen, Formalin-Fixed and Paraffin-Embedded Tissue Sample method

Mifta Rizqina  Amalia; Anna  Roosdiana; Yudit  Oktanella; Andreas Bandang  Hardian; Dini Agusti  Paramanandi; Kharisma Kurnia  Utami; Andi Tri Rakhmat  Akbar; Made Venika  Nareswari; Fajar Shodiq  Permata

doi:10.18006/2022.10(3).643.650

Authors

Mifta Rizqina Amalia Faculty of Veterinary Medicine, Brawijaya University, Puncak Dieng Eksklusif, Kalisongo, Kec. Dau, Kab. Malang 65151, Indonesia
Anna Roosdiana Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Kel. Ketawanggede, Kec. Lowokwaru, Kota Malang, Jawa Timur, 65145, Indonesia
Yudit Oktanella Faculty of Veterinary Medicine, Brawijaya University, Puncak Dieng Eksklusif, Kalisongo, Kec. Dau, Kab. Malang 65151, Indonesia
Andreas Bandang Hardian Faculty of Veterinary Medicine, Brawijaya University, Puncak Dieng Eksklusif, Kalisongo, Kec. Dau, Kab. Malang 65151, Indonesia
Dini Agusti Paramanandi Faculty of Veterinary Medicine, Brawijaya University, Puncak Dieng Eksklusif, Kalisongo, Kec. Dau, Kab. Malang 65151, Indonesia
Kharisma Kurnia Utami Faculty of Veterinary Medicine, Brawijaya University, Puncak Dieng Eksklusif, Kalisongo, Kec. Dau, Kab. Malang 65151, Indonesia
Andi Tri Rakhmat Akbar Faculty of Veterinary Medicine, Brawijaya University, Puncak Dieng Eksklusif, Kalisongo, Kec. Dau, Kab. Malang 65151, Indonesia
Made Venika Nareswari Faculty of Veterinary Medicine, Brawijaya University, Puncak Dieng Eksklusif, Kalisongo, Kec. Dau, Kab. Malang 65151, Indonesia
Fajar Shodiq Permata Faculty of Veterinary Medicine, Brawijaya University, Puncak Dieng Eksklusif, Kalisongo, Kec. Dau, Kab. Malang 65151, Indonesia

DOI:

https://doi.org/10.18006/2022.10(3).643.650

Keywords:

DNA isolation, Electrophoresis, Formalin-fixed, Paraffin-embedded, PCR

Abstract

The purpose of this study was to compare the purity, concentration, and DNA band visualization of the isolated sample and PCR amplicon from three sample storage methods i.e. fresh frozen sample ( -20^oC to -196^oC), preserved in formalin, and paraffin wax. For this tissue samples were collected from the sample stored at frozen temperature -20^oC, 10% NS formalin, and paraffin-embedded preparations, and Abs260/230 and Abs260/280 values and electrophoresis of 0.8% and 2% agarose gel visualization were analyzed. The results of the study showed a significant value of Abs260/280 for the isolated and amplified DNA purity. Among the tested three methods, frozen sample isolates and the PCR amplicon visualized a good DNA band. Meanwhile, the formalin-fixed and paraffinized tissue storage method showed a slightly lower quality DNA and no DNA band, respectively, while the PCR amplicon visualized a thin DNA band. In conclusion, all the tissue storage methods can be applied for DNA preservation and isolation, and the samples are successfully amplified on PCR examination.

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