Morphotaxometry and Ultratopography of Lytocestus haryanii n.sp. (Caryophyllidea: Lytocestidae) from the intestine of freshwater catfish Clarias batrachus Linnaeus 1758 (Siluriformes: Clariidae) of river Yamuna, Yamuna Nagar, Haryana, India
DOI:
https://doi.org/10.18006/2022.10(3).554.566Keywords:
Lytocestus haryanii n. sp., Clarias batrachus, Morphotaxometry, Ultratopography, Histology, MicrotomyAbstract
The present investigation deals with the first report of newer species of caryophyllid cestodes, Lytocestus haryanii n.sp. (Caryophyllidea: Lytocestidae) in the freshwater catfish, Clarias batrachus Linnaeus 1758 (Siluriformes: Clariidae) of river Yamuna from Yamuna Nagar, Haryana, India from July 2018 to June 2020. These helminthes are the most common cestodes (endoparasites) among the fishes of fresh water, brackish water, and marine habitat worldwide. The recovered newer worms were processed through the standardized protocol for the microscopic observations and morphometry, ultratopographic study through scanning electron microscopy, and anatomical analysis by histology using microtomy techniques followed by the double staining. The findings of the present worms were substantiated and compared with the earlier reported species of the same genera from different hosts shared the common group using advanced numerical taxonomy for the taxometric validation. The present proposed newer worms shared all the common characteristics which helped in the generic diagnosis and are closely related to the species collected from the same host species inhabiting different freshwater bodies. The worms comprised several striking contrasts in the combination of distinguishing characters of taxonomic significance in special reference to shape, size, orientation, distribution, and the dimension of the body (single proglottid), scolex, neck, testes, ovary, cirrus pouch, vitellaria, eggs, and excretory pore. Based on the striking morphological, taxometric, ultratopgraphic, and histological differences summarized here can, therefore, be used to propose the worm as a new species.
References
Al–Shehadat, S., Gorduysus, M. O., Abdul Hamid, S. S., Abdullah, N. A., Samsudin, A. R., & Ahmad, A. (2018). Optimization of scanning electron microscope technique for amniotic membrane investigation: A preliminary study. European Journal of Dentistry, 12, 574–578.
Ash, A., Scholz, T., Oros, M., & Kar, P. K. (2011). Tapeworms (Cestoda: Caryophyllidea), parasites of Clarias batrachus (Pisces: Siluriformes) in the Indomalayan region. Journal of Parasitology, 97(3), 435–459.
Ash, A., Scholz, T., Oros, M., Levron, C., & Kar, P. K. (2012). Cestode (Caryophyllidea) of the stinging catfish Heteropneustes fossilis (Siluriformis: Heteropneuestidae) from Asia. Journal of Parasitology, 97(5), 899–907.
Barshe, M. U., Bhure, D. B., & Nanware, S. S. (2018). Morphotaxonomic studies on caryophyllidean cestode genus Lytocestus Cohn, 1908 from freshwater catfish Clarias batrachus with description of new species. Annals of Natural Science, 4(3), 7–19.
Bhavsar, K., Bhangale, A., & Kalse, A. (2020). Reporting a new cryophyllidean worm from a freshwater Clarias batrachus. Journal of Engineering Technology and Research, 7(10), 284–292.
Bhure, D. B., Waghmare, S. B., Kasar, C. R., & Shaikh, K. M. (2010). Taxonomic observation of the caryophyllidean tapeworm Lytocestus Cohn, 1908 from Clarias batrachus (Linnaeus, 1758). Journal of Ecology and Environmental Science, 1(1), 01–06.
Bovien, P. (1926). Caryophyllaeidae from Java. Videnskabelige Meddeleserfra Dansk Naturhistorisk Forening L Kobenhavn, 82, 157–181.
Bray, J. R., & Curtis, J. T. (1957). An ordination of the upland forest communities of Southern Wisconsin. Ecology Monograph, 27, 325–349.
Cain, A. J., & Harrison, G. A. (1958). An analysis of the taxonomists judgment of affinity. Proceedings of Zoological Society London, 13, 85–98.
Cohn, L. (1908). Die Amatomiqeines neuan Fischcestoden. Centrabl Bakt Parasitenk, 46, 134–139.
Dandawate, R. R. (2018). On Lytocestus mulaansis n. sp. from fresh water fish Clarias batrachus from Mula Dam at Baragaon, Nandur, Talukrahauri Dist. Ahmadnagar M.S. Aarhat Multidisciplinary International Education Research Journal, 7(XV), 37–43.
Deshmukh, V. S., Nanware, S. S., & Bhure, D. B. (2015). Biosystematic studies on caryophyllidean cestoda genus Lytocestus from freshwater catfish Clarias batrachus with description of new species. Flora Fauna, 21(2), 179–190.
Dixit, S., Capoor, V. N., & Malhotra, S. K. (1979). A new approach for taxometric application in cestode taxonomy. 2nd National Congress of Parasitology India, Abt. I (32), 7–18.
Furhmann, O., & Bear, J. G. (1925). Zoological results of the third Tanganyika Expendition conducted by Dr. W.A. Cunnigton, 1904–1905. Report on the Cestode. Proceedings of Zoological Society London, 79–100.
Furtado, J. I. (1963). A new caryphyllaeid cestode, Lytocestus parvulus sp. nov. from a Malayan cat fish. Annals and Magazine of Natural History (Ser 13), 6, 93–106.
Furtado, J. I., & Kim–Low, J. (1973). Incidence of some helminth parasites in the Malaysian catfish Clarias batrachus (L.). Verhandlungen Internationale Vereinigung fu¨r Theoretische und Angewandte Limnologie, 18(3), 1674–1685.
Gegenbaur, C. (1859). Gundriss der vergleichenden Anatomie. Leipsig, Germany viii, pp. 660.
Hunter, G. W. (1927). Studies on the caryophyllaeidae of North America. Illinois Biological Monographs, 11, 1–186.
Jadhav, B. V., & Ghavane, A. V. (1991). Two new cestode from Caryophylleidae at Aurangabad. International Journal of Innovative Zoology and Aquatic Biology, 3(1), 28–31.
Jadhav, B. V., Bhure, D. B., & Padwal, N. (2008). Caryophyllidean review from catfishes of Maharashtra (India). Flora Fauna, 14(1), 3–22.
Jawale, S., & Borde, S. (2011). New species of the genus Lytocestus (Caryophyllidea: Lytocestidae) from catfish in Aurangabad District (M.S.), India. International Multidisciplinary Research Journal, 1(8), 27–30.
Jawlikar, J. D., Pawar, S. B., & Shinde, G. B. (2008). A new species Lytocestus subhapradhi n. sp. (Eucestoda: Lytocestidae) from Clarias batrachus. Uttar Pradesh Journal of Zoology, 28(3), 354–369.
Kadam, K. N., & Dhole, J. S. (2011). New tapeworm Lytocestus gariepinusaen. sp. from a freshwater fish Gariepinus at Makani Dam, Dist. Osmanabad, M.S. India. Recent Research in Science and Technology, 3(8), 19–23.
Kadam, M. N., Hiware, C. J., & Jadhav, B. V. (1998). On a new caryophyllid cestode of genus Lytocestus Cohn, 1908 from Clarias batrachus. Dr Bhimrao Ambedkar Marathwada University Aurangabad Journal of Science, 29(6), 143–148.
Kale, S. S. (2017). A new species of cestode Lytocestus paithanensis (Lytocestus Cohn, 1908) from Clarias batrachus at Paithan, M.S. India. International Journal of Life Science, 5(3), 455–458.
Kalse, A. T. & Shinde, G. B. (1999). Lytocestus chalisgaonesis n. sp. (Cestoida: Caryphyllidea) from the catfish Clarias batrachus at Chalisgaon, M.S. India. Rivista Di Parasitologia, 16(60), 39–42.
Kankale, N. M. (2017). A new species of genus Lytocestus ambe from a fresh water fish Clarias batrachus. International Journal of Researches in Biosciences, Agriculture and Technology, 5(2), 242–243.
Kaul, S. S., Kalse, A. T., & Suryawanshi, R. B. (2010). Lytocestus murharii sp. nov. (Cestode:Caryophyllidea) from the catfish Clarias batrachus (L) at Chaalisgaon. Deccon Current Science, 3(1), 73–84.
Khadap, R. M., Jadhav, B. V., & Suryavanshi, N. V. (2004). A new species of the genus Lytocestus (Cohn, 1908), from Clarias batrachus at Aurangabad. National Journal of Life Science, 1(2), 413–416.
Klauber, L. M. (1940). Two new subspecies of Phyllorhynchus, the nosed snake, with notes on genus. Transactions of the San Diego Society of Natural History, 9, 195–214.
Kolpuke, M. N. (1999). On a new species of the genus Lytocestus Cohn, 1908 (Cestode: Caryphyllidea) from Wallago attu from Terna river at Aurangabad, India. Uttar Pradesh Journal of Zoology, 19(1), 93–95.
Kumar, S., Jaiswal, N., Malhotra, A., Upadhyay, S. K., Capoor, V. N., & Malhotra, S. K. (2011). Application of numerical taxonomy for morphological and taxometric substantiation of cystidicolid nematodes, Ascarophis alatae n.sp. from Indian eels. Proceedingss of the Zoological Society of India (Spl. Issue– Parasitology), 1, 45–50.
Lakhe, A. D., Pawar, S. B., Shinde, G. B., & Patil, A. S. (2004). A new cestode Lytocestus nagapurensis n. sp. (Cotyloda– Lytocestidae). Rivista Di Parasitologia, 11, 95–98.
Linnaeus, C. (1758). Systema naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Tomus I. Editio decima, reformata. Homiae: Laurentii Salvii.
Lynsdale, J. A. (1956). On two new species of Lytocestus from Burma and Sudan respectively. Journal of Helminthology, 30(2–3), 87–96.
Malhotra, A., Jaiswal, N., Malakar, A. K., Verma, M. S., et al. (2012). The morphology and genetic characterization of Iheringascaris goaii n.sp. (Nematoda: Raphidascarididae) from the intestine of the silver whiting and spotted catfish of the central west coast of India. Journal of Helminthology, 86(3), 353–362.
Malhotra, S. K., Capoor, V. N., & Shinde, G. B. (1981c). Introduction of a taxonometric device to evaluate a new proteocephalid cestode Gangesia sanehensis from freshwater fishes of Garhwal with a revised key to species of the genus Gangesia. Marathwada University Journal of Science, 19(12), 43–52.
Malhotra, S. K., Dixit, S., & Capoor, V. N. (1981a). Taxometric evaluation of a fish cestode Gangesia mehamdabadensis n. sp. from Mystus tengra from Mehamdabad, Gujrat. Science and Culture, 3(1), 7–20.
Malhotra, S. K., Dixit, S., & Capoor, V. N. (1981b). A contribution to the study of taxa differentiation in cestode taxonomy. Proceedings of Indian Academy of Science (Animal Science), 90(3), 343–349.
Mehra, H. R. (1930). On a new species of caryophyllid of Muller from Kashmir with remarks on Lytocestus indicus (Moghe, 1925). Proceedings of 17th Indian Science Congress, pp. 247.
Moghe, M. A. (1925). Caryophyllaeus indicus n. sp. (cestode) from the catfish (Clarias batrachus) (L.). Parasites, 17, 232–235.
Moghe, M. A. (1931). A supplementary description of Lytocestus indicus Moghe (Syn. Caryophyllaeus indicus Moghe, 1925 Cestoda). Parasites, 23, 84–87.
Murhar, B. M. (1963). Cresentovitus biloculus gen. sp. A fish cestode (Caryophyllaeidae) from Nagpur, India. Parasites, 53, 413–418.
Narayan, A., & Srivastav, A. K. (2020). Study of Lytocestus species of the edible catfish Heteropneustes fossilis from central India. Pakistan Journal of Parasitology, 70, 23–27.
Nimbalkar, R. K., Deolalikar, R. V., & Muleu, S. P. (2012). Study on a new of Lytocestus (Cohn, 1908) from Heteropnuestus fossilis ( Bloch) at Jaikwadi Dam of Aurangabad district M.S. Life Science Bulletin, 9(2), 239–242.
Pardeshi, K. S. (2016). Cestode Lytocestus mastacembellusi. International Journal of Scientific Research and Educational Research, 4(4), 5140–5143.
Patil, D. N., & Jadhav, B. V. (2002). On a new caryophyllid cestode of the genus Lytocestus Cohn, 1908, from Clarias batrachus. Indian Journal of Helminthology (NS), 20, 45–48.
Patil, D. P. (2018). A new species of the genus Lytocestus (Caryophyllida: Lytocestidae) from Clarias batrachus. Review Research, 7(6), 1–4.
Pawar, R. G., & Dandwate, R. R. (2013). Lytocestus godavarensis new spp. From Clarias batrachus (Linnus, 1758) at Pravarasangam Dist. Ahmednagar, India. Deccan Current Science, 9(1), 183–187.
Pawar, S. B., & Hiware, C. J. (2011). Two new species of the genus Lytocestus (Caryophyllidea: Lytocestidae) from fresh water catfish Clarias batrachus (Linnaeus, 1758). Recent Research in Science and Technology, 3(12), 25–28.
Pawar, S. B., & Shinde, G. B. (2002). A new species Lytocestus batrachusae n. sp. (Cotyloda– Lytocestidae) from Clarias batrachus at Aurangabad, India. Rivista Di Parasitologia, 19, 153–156.
Pearse, A. G. E. (1968). Histochemistry: Theoretical and applied. London: Churchill. vol. 2, pp.530.
Poonam, (2007). On a new species of the genus Lytocestus (Caryophyllidea– Lytocestidae) from Clarias batrachus. Proceedingss of the Zoological Society of India, 6(1), 77–81.
Ramadevi, P. (1973). Lytocestus longicollis sp. nov. (Cestoda: Caryophylliaeda) from cat fish, Clarias batrachus in India. Journal of Helminthology, 47, 415–420.
Sahay, U., & Ekka, P. (2019). On the status of Lytocestus jagtai (Caryophyllidea: Lytocestidae) Tripathi Singh & Mishra 2007– a critical study. Trends in Fisheries Research, 8(2), 78–85.
Sahay, U., & Khalkho, A. P. V. (2017). A discussion on the status of Lytocestus rekhaensis Nimbalkar et. al., 2012. Biospectra, 12(1), 1–8.
Sahay, U., Khalkho, A. P. V., Ekka, P., & Mandal, D. (2019a). The existence of Lytocestus paithanensis Kale, 2017 is questionable– A critical study. Trends in Fisheries Research, 8(2), 62–68.
Sahay, U., Khalkho, A. P. V., Singh, R. R., & Mandal, D. (2019b). Status of Lytocestus mastacembellusi (Caryophyllidea: Lytocestidae) Pardedhi, 2016– A critical study. Asian Journal of Agriculture and Life Sciences, 4(10), 13–21.
Sahay, U., Mandal, D., Saxena, N., & Singh, R. R. (2017). On the validity of Lytocestus heteropneustii (cestoda) Tandon, Chakravarty and Das, 2005– A critical review. Biospectra, 12(2), 115–120.
Sahay, U., Singh, R. R., & Saxena, K. (2018b). On the status of Lytocestus indica (Lytocestida: Caryophyllidea) Deshmukh et al. 2015 – A critical study. Trends in Parasitology Research, 7(1), 1–7.
Sahay, U., Singh, R. R., Kamal, S., & Jha, A. (2018a). On resurrection of certain Lytocestus species (Caryophyllidae: Lytocestidea) showing granular vitellaria– A critical study. Journal of Experimental Zoology, 21(2), 1271–1276.
Sawarkar BW (2012). Record of new tapeworm Lytocestus alii n. sp. from freshwater fish Clarias batrachus (Bleeker, 1862) at Amravati, Maharashtra, India. Journal of Boilogy & Life Sciences, 3(1), 281–287.
Sawarkar, B. W., & Kale, G. B. (2012). New tapeworm Lytocestus thapari n. sp. from a freshwater fish Clarias batrachus (Bleeker, 1862) at Aurangabad, Maharashtra, India. UGC Sponsored National Conference on Recent Trends in Biosciences, 3(1), 281–287.
Shelke, V. P. (2007). Lytocestus paithanensis n. sp. from Clarias batrachus. National Journal of Life Sciences, 4(3), 151–152.
Shinde, G. B., & Phad, A. N. (1988). On a new cestode Lytocestus marathwadensis from fresh water fish. Revista Di Parasitologia, 47(2), 295–298.
Shinde, G. B., & Borde, S. (1999). On Lytocestus kopardaensis n. sp. cestode Lytocestidae Hunter from a fish in Maharashtra state, India. Uttar Pradesh Journal of Zoology, 19(3), 211–213.
Shinde, G. B., & Deshmukh, R. A. (1980). Redescription of two species of the genus Lytocestus Cohn, 1908 (Cestoda: Caryophyllidea) from fresh water fish. Revista Di Parasitologia, XLI(2), 209–214.
Shinde, G. B., & Pawar, S. B. (2002). A new species Lytocestus clarisae n. sp. Revista Di Parasitologia, XIX(LXIII), 157–160.
Shomendra, M., Jha, A. N., & Kumar, P. (2003). A new cestode Lytocestus bishnupurensis from a fresh water fish Mystus seenghala (Sykes). Journal of Freshwater Biology, 15(1–4), 43–45
Singh, R. R., Sahay, U., & Sadaf, F. (2018). On the synonymy of Lytocestus bishnupurensis Shomendra et al. (2003) with L. indica Moghe (1925). Journal of Experimental Zoology, 21(2), 893–896.
Singh, S. S. (1975). On Lytocestus fossilis n. sp. (Cestoda: Lytocestidae) from Heteropneustus fossilis from Nepal. In K.K. Tiwari, C.B. Srivastava (eds.) B.S. Chauhan Commemoration Volume, 1975, Orissa, India. Prooceedings of Zoological Society of India, pp. 79–82.
Solunke, R., Fadke, S., Borde, S., & Jawale, S. (2012). New species of the genus Lytocestus (Caryophyllidea: Lytocestidae) from catfish in Latur dist. (M.S.), India. Trends in Parasitology Research, 1(2), 25–30.
Surayawanshi, S. G., Maske, D. K., Shinde, G. B., & Bhagwan, H. K. (2010). A new tapeworm Lytocestus shindein. sp. (Cestoda; Lytocestidae) from Clarias batrachus at Rahuri Dist. Ahmednagar (M.S.). Life Science Bulletin, 1, 148–150.
Tandon, V., Chakravarty, R., & Das, B. (2005). Four new species of the genus Lytocestus (Caryophyllidea: Lytocestidae) from edible catfishes in Assam and Meghalaya, India. Journal of Parasitic Diseases, 29(2), 131–142.
Tripathi, N. P., Singh, S. P., & Mishra, A. K. (2007). A new species of the genus Lytocestus (Cestoda: Lytocestiodae) from Heteropneustes fossilis at Rewa (M.P.). National Journal of Life Sciences, 4(3), 111–114.
Upadhyay, S. K. (2012). Transmission dynamics and environmental influence on food borne parasitic helminthes of the Gangetic plains and Central West coast of India. Unpublished D.Phil Thesis, University of Allahabad, India.
Upadhyay, S. K. (2017). Histopathological and distribution aspects of natural enoplid infections in hepatic tissue of wild rat, Rattus rattus in Allahabad. Indian Journal of Helminthology (N.S.), 36 & 37, 51–55
Upadhyay, S. K. (2018). Morphotaxonomy, histopathology and population dynamics of natural enoplid infections in wild rat, Rattus rattus of eastern Uttar Pradesh, India. AASCIT Journal of Bioscience, 4(3), 22–27.
Upadhyay, S. K. (2019). Morphotaxometry of a new roundworm Heterakis equispiculis n.sp. (Nematoda: Heterakidae) from rodents of Bundelkhand region at Uttar Pradesh. Proceedingss of the Zoological Society, 72(2), 171–179.
Upadhyay, S. K., & Nanware, S. S. (2020). Parasitoses and histological consequences of Trichuris trichiura (Nematoda: Enoplida) in rodents, Rattus rattus (Mammalia: Rodentia). Asian Journal of Biological and Life Sciences, 9(1), 74–78.
Upadhyay, S. K., Jaiswal, N., Malhotra, A. & Malhotra, S.K. (2009). An aspidoderid round worm Pseudaspidodera cordinae n.sp. from rodents at Allahabad. Indian Journal of Helminthology (N.S.), 27, 89–94.
Upadhyay, S. K., Jaiswal, N., Malhotra, A., & Malhotra, S. K. (2013). Ecological morphotaxometry of trematodes of garfish (Teleostomi: Belonidae) from Gangetic riverine ecosystem in India. I. Morphology and taxometric assessment of by Cephalogonimus yamunii n.sp. Journal of Parasitic Diseases, 37(2), 218–224.
Wardle, R. A., & McLeod, J. A. (1952). The Zoology of tapeworms. University of Minnesota Press, Minneapolis, pp. 780.
Wisse, E., Braet, F., Duimel, H., Vreuls, C., Koek, G., Olde Damink, S.W.M., et al. (2010). Fixation methods for electron microscopy of human and other liver. World Journal of Gastroenterology, 16(23), 2851–2866
Woodland, W. N. F. (1923). On some remarkable new forms of Caryophyllaeidae from the Anglo– Egyption Sudan and a revision of the families of the Cestodaria. Quarterly Journal of Microbiology, 67, 435–472.
Woodland, W. N. F. (1924).On a new genus of Proteocephalidae from Indian fresh water fishes. Parasitology, 16, 441–451.
Woodland, W. N. F. (1926). On the genera and possible affinities of Carryophyllaeidae: A reply to Dr. O. Fuhrmann and J. G. Bear. Proceedings of Zoological Society London, pp. 49–69.
Yamaguti, S. (1959). Studies on the helminth fauna of Japan. IV. Cestodes of fishes. Japanese Journal of Zoology, 6, 1–112
Yamaguti, S. (1961). Systema Helminthum. Volume II. The Cestodes of Vetebrates. (Part II) Interscience Publications Inc NY. 860p.
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