GENOME-WIDE IDENTIFICATION OF THE GATA TRANSCRIPTION FACTOR FAMILY IN Dichanthelium oligosanthes

Satyabrata  Nanda; Gagan  Kumar; Sudheer Kumar  Yadav; Sajid  Hussain

doi:10.18006/2021.9(4).407.416

Authors

Satyabrata Nanda MS Swaminathan School of Agriculture, Centurion University of Technology and Management, Odisha, India. https://orcid.org/0000-0001-9729-1788
Gagan Kumar MS Swaminathan School of Agriculture, Centurion University of Technology and Management, Odisha, India.
Sudheer Kumar Yadav Narayan Institute of Agriculture Sciences, Gopal Narayan Singh University, Bihar, India.
Sajid Hussain State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.

DOI:

https://doi.org/10.18006/2021.9(4).407.416

Keywords:

GATA, Transcription Factors, Genome-wide identification, C3 plants, Dichanthelium oligosanthes

Abstract

The GATA transcription factors (TFs) play a crucial role in regulating various physiological processes in plants. Identification and characterization of the GATA TF family has been carried out in several important grass species, including rice, maize, and bamboo. However, no information is available on the GATA TFs in the C₃ grass species Dichanthelium oligosanthes. In the current study, 31 GATA genes have been identified in the D. oligosanthes genome by stringent bioinformatics analysis. The exon-intron arrangement analysis of the DoGATAs via the Gene Structure Display Server (GSDS 2.0) revealed the redundancy and differences in their gene structural organization. In addition, the sequence comparisons within the DoGATAs via BLAST revealed 11 numbers of putative paralogs. Similarly, the BLAST comparisons among the OsGATAs and DoGATAs resulted in the identification of 21 orthologs. Structural analysis of the identified DoGATAs through Simple Modular Architecture Research Tool (SMART), Conserved Domain Database (CDD), and Multiple Expectation Maximization for Motif Elicitation (MEME) revealed that all of them possess the signature GATA domain and the C-X₂-C-X₁₈-C-X₂-C consensus sequence. The phylogenetic analysis via MEGA divided the DoGATAs into four groups along with rice and Arabidopsis GATAs. In addition, the subcellular localization, gene ontology, and other peptide functional prediction results further supported the DoGATAs to be putative GATA genes. Moreover, the findings of this study can serve as a basic framework for the isolation and functional characterization of GATA genes in D. oligosanthes.

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