Ameliorating Direct Blue Dye Degradation Using Trametes versicolor Derived Laccase Enzyme Optimized through Box–Behnken Design (BBD) via Submerged Fermentation

Umamaheswari Ramaswamy; Ramkumar Lakshmanan; Mythili Ravichandran; Prabu Periasamy; Shanmugam Sengodan

doi:10.18006/2022.10(4).818.830

Authors

Umamaheswari Ramaswamy Department of Microbiology, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Namakkal, India
Ramkumar Lakshmanan Department of Microbiology, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Namakkal, India
Mythili Ravichandran Department of Microbiology, Vivekanandha Arts and Science College for Women, Sankari, Namakkal, India
Prabu Periasamy Department of Biotechnology, Periyar University PG Extension Center, Dharmapuri, India
Shanmugam Sengodan Department of Microbiology, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Namakkal, India

DOI:

https://doi.org/10.18006/2022.10(4).818.830

Keywords:

Laccase, Trametes versicolor, Fruit peels, Dye decolorization, Box-Behnken design

Abstract

The major intend of this study was to elucidate the laccase production by Trametes versicolor under submerged fermentation using fruit waste peel as substrate. The textile dye was decolorized by the procured crude enzymatic extract using the response surface methodology. The submerged media with organic fruit peel waste extract (jackfruit, pineapple & kaffir) supplemented with gypsum, calcium carbonate, and nutrient broth were considered superior for laccase production. The produced laccase enzyme was used in dye decolorization at the optimum conditions using the Box-Behnken design. Subsequently, the experiment was designed with four variables (dye concentration, pH, temperature & time) with three factors to achieve the maximum direct blue dye decolorization. The highest laccase activity level was obtained from jackfruit peel extract with 3.86U/ml on 15^th day at 25^oC with pH 5.0 when compared to the other two extracts. The maximum laccase activity with guaiacol was obtained at optimum pH 4 and 40^oC. The predicted value was experimentally validated by attaining 81.25% of dye color removal. From the result, the optimum conditions for direct blue color removal were: dye concentration 40ppm, pH 4.0, temperature 40^oC at 24 hours. From the results of this study, it was concluded that the jack fruit peel was a more suitable substrate for laccase production. The dye decolorization results were recommended that Box-Behnken design for parameters optimization. The T. versicolor laccase was more proficient for textile dye decolorization. The opportunity was created by using the laccase enzyme for the biological treatment of textile dyeing effluent before discharging into the environment.

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