IN VIVO AND IN VITRO EVALUATION OF PHARMACOLOGICAL ACTIVITIES OF Hedychium coccineum RHIZOMES EXTRACT

Sadia Islam  Nishi; Niloy  Barua; Mohammed Aktar  Sayeed; Abu Montakim  Tareq; Sahnaj Begum  Mina; Talha Bin  Emran; Kuldeep  Dhama

doi:10.18006/2021.9(3).335.342

Authors

Sadia Islam Nishi Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh
Niloy Barua Drug Discovery, GUSTO A Research Group, Chittagong 4203, Bangladesh
Mohammed Aktar Sayeed Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh
Abu Montakim Tareq Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh https://orcid.org/0000-0003-2704-7610
Sahnaj Begum Mina Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh
Talha Bin Emran Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh https://orcid.org/0000-0003-3188-2272
Kuldeep Dhama Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, Uttar Pradesh, India https://orcid.org/0000-0001-7469-4752

DOI:

https://doi.org/10.18006/2021.9(3).335.342

Keywords:

Hedychium coccineum, Zingiberaceae, Antidiarrheal, Anthelmintic, Thrombolytic

Abstract

The study reports the in vivo antidiarrheal and in vitro anthelmintic, cytotoxic, and thrombolytic activity of methanol extract of Hedychium coccineum rhizomes (MEHC). The antidiarrheal activity was determined using Castor oil-induced diarrhea and Gastrointestinal motility test in mice at the doses of 200 and 400 mg/kg body weight, whereas an aquarium worm, Tubifex tubifex, was used to determine the anthelmintic activity. The cytotoxic and thrombolytic activity of MEHC was performed by Brine shrimp lethality bioassay and clot lysis method respectively. In antidiarrheal, castor oil-induced diarrhea and gastrointestinal motility exhibited a significant reduction in diarrhea and defecation and an extremely significant inhibition in intestinal motility and peristalsis index by 200 and 400 mg/kg of MEHC. The MEHC (5, 10, and 20 mg/mL) showed a significant dose-dependent manner paralysis time and times to death in multiple comparisons to the different levamisole concentrations (0.5, 0.8, and 1 mg/mL) at in vitro anthelmintic activity. The brine shrimp lethality bioassay exhibited a weak LC₅₀(681.95 µg/mL; R² = 0.951) while in thrombolytic a significant percentage of clot lysis (32.70%, P < 0.05) demonstrated. The findings demonstrate that H. coccineum rhizomes could be potential sources for biological activity.

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