EFFECT OF Staphylococcus epidermidis ON Pseudomonas aeruginosa BIOFILM IN MIXED-SPECIES CULTURE

Suhaga  Dohare; Devendra  Singh; Deepmala  Sharma; Vishnu  Agarwal

doi:10.18006/2021.9(3).325.334

Authors

Suhaga Dohare Department of Biotechnology Motilal Nehru National Institute of Technology Allahabad-211004, India
Devendra Singh Department of Biotechnology Motilal Nehru National Institute of Technology Allahabad-211004, India
Deepmala Sharma Department of Mathematics, National Institute of Technology, Raipur-492010, India
Vishnu Agarwal Department of Biotechnology Motilal Nehru National Institute of Technology Allahabad-211004, India

DOI:

https://doi.org/10.18006/2021.9(3).325.334

Keywords:

Staphylococcus epidermidis, Pseudomonas aeruginosa, Mixed culture, Planktonic, Biofilm, Co-survivability

Abstract

Staphylococcus epidermidis and Pseudomonas aeruginosa, are clinically relevant pathogens that often produce biofilms. To investigate the co-survivability of S. epidermidis and P. aeruginosa in mixed cultures biofilm and planktonic form, it is important to understand more about the interspecies interaction of both species. The interspecies interaction was analyzed using streak and drop agar plate assay, cell viability assay (CFU), spectrophotometry-based method, and microscopic analysis. The findings suggest that both cells and supernatant of P. aeruginosa inhibit the planktonic growth of S. epidermidis. The cell viability result shows that PAO1 biofilm cells were decreased by 88%, and SE biofilm cells were increased by 75% concerning their control. Opposite to the P. aeruginosa, the S. epidermidis biofilm and EPS matrix were found to increase in mixed culture biofilm, which was further confirmed by microscopic analysis. In contrast, differential agar media result shows that the reduction in the biofilm (CFU/ml) of P. aeruginosa is independent of S. epidermidis cells concentration. Finally, the effect of the supernatant on biofilm was investigated, and it found that S. epidermidis biofilm was enhanced while P. aeruginosa biofilm was reduced in the presence of partner bacterial supernatant, which indicated that S. epidermidis in biofilm mode could hinder the biofilm formation of P. aeruginosa. The outcomes show that the culture supernatant of S. epidermidis can be used to prevent P. aeruginosa associated biofilm infections.

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