A detailed investigation to study the pattern of the interplay of Cyclic AMP Receptor Protein (CRP) of E. coli with its different classes of promoters

Soumyajit  Chandra; Saikat  Samanta; Rameswar  Mukhopadhyay; Rajib  Majumder; Abinit  Saha

doi:10.18006/2022.10(1).266.277

Authors

Soumyajit Chandra School of Life Science and Biotechnology, Adamas University, Kolkata 700126, West Bengal, India https://orcid.org/0000-0002-2227-8279
Saikat Samanta School of Life Science and Biotechnology, Adamas University, Kolkata 700126, West Bengal, India https://orcid.org/0000-0001-9694-787X
Rameswar Mukhopadhyay School of Life Science and Biotechnology, Adamas University, Kolkata 700126, West Bengal, India https://orcid.org/0000-0003-1007-2000
Rajib Majumder School of Life Science and Biotechnology, Adamas University, Kolkata 700126, West Bengal, India https://orcid.org/0000-0001-9069-2319
Abinit Saha School of Life Science and Biotechnology, Adamas University, Kolkata 700126, West Bengal, India https://orcid.org/0000-0003-1590-8073

DOI:

https://doi.org/10.18006/2022.10(1).266.277

Keywords:

Biphasic nature, Transcription activation, CAP, CRP, AR1, AR2, AR3

Abstract

The activity of most of the promoters in Escherichia coli, involved in the metabolism of sugars other than glucose, is controlled by a CRP (cAMP receptor protein) or CAP (catabolite activator protein). CRP-dependent promoters are differentiated into various classes (Class I, Class II, and Class III) based on its cognate binding site’s position on DNA. The promoters regulated by CAP are differentially regulated by this transcriptional factor and it is also imperative to mention that these promoters vary greatly in respect to the binding site of CAP to its cognate binding site, it has also been reported that either it overlaps with the binding site of RNA polymerase or it present upstream to it. In Class I CAP-dependent promoters, a particular CAP molecule makes protein-protein interaction for the start of transcription. In Class II CAP-dependent promoters, a particular CAP molecule makes multiple interactions for the start of transcription. At last, in Class III-CAP dependent promoters, more than one CAP molecule is involved and activation of transcription is done synergistically. It has also been documented that CAP shows a kind of biphasic behavior in some promoters. So, the main focus of this work is to find out whether this biphasic behavior is true for other E. coli promoters as well. Experiments have been performed to know more about this biphasic nature and the various patterns of interactions of catabolite activator protein (CAP) of E. coli with its different classes of promoters.

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