Phylogenetic and Morphological Study of Desmodesmus Strains from Can Gio Mangrove Biosphere Reserve
DOI:
https://doi.org/10.18006/2024.12(6).829.837Keywords:
Microalgae, Can Gio Biosphere Reserve, ITS, 18SAbstract
This study focused on five microalgal strains resembling Desmodesmus isolated from the Can Gio Mangrove Biosphere Reserve. The objective was to assess the effectiveness of morphological and molecular methods for algal identification and to evaluate the genetic diversity of the relevant taxa. The five isolated and reference strains were cultured axenically in a BG-11 medium. Both microscopy (at magnifications of 40× and 100×) and molecular techniques (using ITS and 18S rRNA markers) were employed for analysis. Phylogenetic analyses were conducted using Maximum Likelihood and Bayesian inference methods. The results indicated that five of the ten strains were consistently identified using both approaches. Molecular data prompted a taxonomic reassignment for the three remaining strains, while morphological traits were more decisive for two reference strains. Phylogenetic analyses revealed significant genetic diversity within Desmodesmus, highlighting the ecological adaptability of genetically distinct variants. This study emphasizes the reliability of molecular tools in algal taxonomy, particularly for differentiating between Desmodesmus and Scenedesmus-like taxa. It contributes to understanding microalgal genetic diversity in the Can Gio Biosphere Reserve and provides a foundation for future ecological and biotechnological applications.
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