Optimal condition for Propagation and Growing of Dendrobium thyrsiflorum

Nguyen Thi Kim  Cuc; Nguyen Thi  Oanh; Nguyen Thi  Diem; Nguyen Huu  Tho

doi:10.18006/2022.10(3).524.532

Authors

Nguyen Thi Kim Cuc Institute of Biotechnology, Hue University, Road 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam https://orcid.org/0000-0002-1313-6092
Nguyen Thi Oanh Institute of Biotechnology, Hue University, Road 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam https://orcid.org/0000-0001-6295-3809
Nguyen Thi Diem Institute of Biotechnology, Hue University, Road 10, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam https://orcid.org/0000-0002-5035-4793
Nguyen Huu Tho College Electro-Mechanics, Construction, and Agro-Forestry of Central Vietnam, QL1A, Cat Hanh, Phu Cat, Binh Dinh, Vietnam https://orcid.org/0000-0002-0832-8540

DOI:

https://doi.org/10.18006/2022.10(3).524.532

Keywords:

Acclimatization, Dendrobium thyrsiflorum, In vitro, Medicinal orchid, Propagation

Abstract

Dendrobium thyrsiflorum is a medicinal orchid that is being gradually destroyed due to over-exploitation. This research focuses on in vitro production of D. thyrsiflorum plantlet under optimal acclimatization conditions. The results of the study showed that the best medium for seed germination was MS basal medium (full-strength MS nutrient plus 100 ml L^-1 coconut water; 7 g L^-1 agar, 30 g L^-1 sucrose, and 0.5 g L^-1 active charcoal) supplemented with 60 g L^-1 mashed sweet potatoes, which induces 97.8% of seed germination. The shoot was well developed in MS basal medium supplemented with 60 g L^-1 mashed sweet potatoes and 0.4 mg L^-1 6-Benzylaminopurine (BA) and, corpulent and green in shoot morphology. The shoot multiplication rate was greatest on MS basal medium supplemented with 0.4 mg L^-1 BA and 0.4 mg L^-1 Kinetin with 4.53 times, and the shoot height was reported at 3.45 cm after 8 weeks of subculture. Further, The shoot was 100% rooting, with an average of 4.51 roots/shoot and 5.34 cm per root in length when the shoot was implanted on MS basal medium plus 60 g L^-1 mashed sweet potatoes and 0.75 g L^-1 active charcoal. Especially, plantlets after transplanting to the orchid net house reached a 94.8% survival rate on tree fern after 24 weeks. Hence, the results of the study suggested a successful production of D. thyrsiflorum plantlet on the selected media compositions.

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