Optimized in vitro micropropagation and microtuber production in potato (Solanum tuberosum L.) through apical buds using hormone regulation and tissue culture techniques

Authors

  • Arile Ariste Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico. https://orcid.org/0009-0000-7459-6298
  • Ma. del Carmen Ojeda Zacarías Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico. https://orcid.org/0000-0002-0003-3705
  • Héctor Lozoya Saldaña Autonomous University of Chapingo, Phytotechnics Department, Mexico-Texcoco highway Km 38.5, 56230 Chapingo, P.C 56230. Mexico. https://orcid.org/0000-0001-5451-9981
  • Emilio Olivares Sáenz Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico. https://orcid.org/0000-0003-1881-0186
  • Eduardo A. García Zambrano Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico. https://orcid.org/0000-0002-9617-3912
  • Alejandro Ibarra López Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico. https://orcid.org/0000-0003-0515-7544
  • Alhagie K. Cham University of Tennessee Institute of Agriculture, Department of Entomology & Plant Pathology, 2505, 370 E J. Chapman Dr Plant Biotechnology Building, Knoxville, TN 37996, USA. https://orcid.org/0000-0003-2695-9706

DOI:

https://doi.org/10.18006/2025.13(1).86.96

Keywords:

Benzylaminopurine (BAP), Gibberellic acid (GA3), In vitro organogenesis, Micropropagation, Hormone optimization, Microtuber production

Abstract

Potato is an essential crop worldwide, and optimizing micropropagation techniques is important for enhancing germplasm conservation and large-scale production. This study focuses on the in vitro propagation of two potato varieties, Agata and Fianna, emphasizing optimizing sterilization protocols, shoot induction, rooting, and microtuber production. Apical buds from healthy, disease-free plants were selected as explants. These buds were surface-sterilized using 70% ethanol and sodium hypochlorite (NaOCl) with Tween-20. The explants were excised from tuber sprouts and cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of plant growth regulators, including benzylaminopurine (BAP) at 0.10–0.40 mg/L, gibberellic acid (GA3) at 0.20–1.00 mg/L, and naphthalene acetic acid (NAA) at 0.01 and 0.04 mg/L to promote root development. The study also explored the effects of these hormonal treatments on shoot induction, proliferation, and rooting. Significant differences were observed between the two varieties regarding oxidation, contamination, and aseptic conditions, with Fianna demonstrating better resistance to oxidation and contamination than Agata. Shoot multiplication was most efficient with BAP concentrations of 0.40 mg/L for Fianna and 0.30 mg/L for Agata. Root induction was maximized with higher sucrose concentrations (110 g/L) and GA3 (1.00 mg/L) for Agata, while moderate concentrations of these compounds produced optimal results for Fianna. Microtuber formation was most successful with moderate sucrose (80–100 g/L) and GA3 (0.25–0.75 mg/L) concentrations. This study provides valuable insights into optimizing tissue culture practices for potato propagation, enhancing both microtuber production and the overall efficiency of potato production systems.

Author Biographies

Arile Ariste, Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico.

Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico.

Ma. del Carmen Ojeda Zacarías, Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico.

Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico.

Héctor Lozoya Saldaña, Autonomous University of Chapingo, Phytotechnics Department, Mexico-Texcoco highway Km 38.5, 56230 Chapingo, P.C 56230. Mexico.

Autonomous University of Chapingo, Phytotechnics Department, Mexico-Texcoco highway Km 38.5, 56230 Chapingo, P.C 56230. Mexico.

Emilio Olivares Sáenz, Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico.

Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico.

Eduardo A. García Zambrano, Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico.

Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico.

Alejandro Ibarra López, Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico.

Autonomous University of Nuevo León, Faculty of Agronomy, Street Francisco Villa s/n, Col. Ex Hacienda El Canada, Gral. Escobedo, Nuevo León. P.C. 66054. Mexico.

Alhagie K. Cham, University of Tennessee Institute of Agriculture, Department of Entomology & Plant Pathology, 2505, 370 E J. Chapman Dr Plant Biotechnology Building, Knoxville, TN 37996, USA.

University of Tennessee Institute of Agriculture, Department of Entomology & Plant Pathology, 2505, 370 E J. Chapman Dr Plant Biotechnology Building, Knoxville, TN 37996, USA.

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2025-03-05

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Ariste, A., Ojeda Zacarías, M. del C., Lozoya Saldaña, H., Olivares Sáenz, E., García Zambrano, E. A., Ibarra López, A., & Cham, A. K. (2025). Optimized in vitro micropropagation and microtuber production in potato (Solanum tuberosum L.) through apical buds using hormone regulation and tissue culture techniques. Journal of Experimental Biology and Agricultural Sciences, 13(1), 86–96. https://doi.org/10.18006/2025.13(1).86.96

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Vol. 13 No. 1 (2025)

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RESEARCH ARTICLES

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