DECIPHERING THE PHARMACOLOGICAL INSIGHTS OF FRACTIONATED ELATOSTEMA PAPILLOSUM WED. AND HOLIGARNA LONGIFOLIA ROXB. THROUGH IN VITRO AND IN VIVO STUDIES

Md. Zia  Uddin; Md. Sohel  Rana; Subrata  Chowdhury; Arkajyoti  Paul; Shahadat   Hossain; Talha Bin  Emran; Kuldeep  Dhama

doi:10.18006/2021.9(2).189.199

Authors

Md. Zia Uddin Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh https://orcid.org/0000-0003-4767-9279
Md. Sohel Rana Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh https://orcid.org/0000-0003-2298-5982
Subrata Chowdhury Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh
Arkajyoti Paul Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh https://orcid.org/0000-0002-2767-2646
Shahadat Hossain Atomic Energy Centre, East Nasirabad, Chittagong-4209, Bangladesh https://orcid.org/0000-0003-3470-1422
Talha Bin Emran Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4381, Bangladesh https://orcid.org/0000-0003-3188-2272
Kuldeep Dhama Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, Uttar Pradesh, India https://orcid.org/0000-0001-7469-4752

DOI:

https://doi.org/10.18006/2021.9(2).189.199

Keywords:

Holigarna longifolia, Elatostema papillosum, Antibacterial, Anti-arthritic, Hyperglycemia

Abstract

The present research intended to explore the biological activities, namely acute toxicity test and hypoglycemic as well as in vitro anti-arthritic along with the antibacterial activity of crude methanol extracts with its different soluble fractions like petroleum ether (PESF), carbon tetrachloride (CTCSF), chloroform (CSF) and aqueous soluble fraction (AQSF) of Holigarna longifolia and Elatostema papillosum. Phytochemical screening was performed by established protocols. Acute toxicity and hypoglycemic effects were performed in experimental and alloxan-induced diabetic rats. In vitro anti-arthritic and antibacterial activity were conducted by protein denaturation inhibitory and disc diffusion methods. It was observed that no rats exhibit any lethality types, which reveal the safety of plant fractionates. It was also seen that both plants' fractionates showed significant (p < 0.01) activity on hyperglycemia compared to standard. Upon investigation, it was observed that crude methanol and its CS fraction of E. papillosum and only CS fraction of H. longifolia significantly (p < 0.05) inhibited denaturation of bovine serum albumin protein compared to standard diclofenac sodium. Moreover, it was observed that crude methanol extract and its CS fraction of E. papillosum showed significant inhibitory action on all Gram-positive bacteria's growth. In contrast, the PES fraction highlighted an inhibitory zone of 26.7 and 24.7 mm, respectively, towards B. subtilis and S. aureus. This study provides some support to explain the traditional uses of H. longifolia and E. papillosum.

References

Ahmed S, Rakib A, Islam MA, Khanam BH, Faiz FB, Paul A, Chy MNU, Bhuiya NMMA, Uddin MMN, Ullah SMA, Rahman MA, Emran TB (2019) In vivo and in vitro pharmacological activities of Tacca integrifolia rhizome and investigation of possible lead compounds against breast cancer through in silico approaches. Clinical Phytoscience 5:36. DOI: 10.1186/s40816-019-0127-x.

Akter S, Shah M, Tareq AM, Nasrin MS, Rahman MA, Babar ZM, Haque MA, Royhan MJ, Mamun MN, Reza ASMA, Emran TB (2020) Pharmacological effect of methanolic and hydro-alcoholic extract of Coconut endocarp. Journal of Advanced Biotechnology and Experimental Therapeutics 3(3): 171-181.

Aruna RV, Ramesh B, Kartha VN (1999) Effect of betacarotene on protein glycosylation in alloxan induced diabetic rats. Indian Journal of Experimental Biology b(4):399-401.

Aruoma OI (1998) Free radicals, oxidative stress, and antioxidants in human health and disease. Journal of the American Oil Chemists' Society 75: 199-212.

Arya D, Meena M, Neha G, Vidya P (2014) In vitro anti-inflammatory and anti-arthritic activity in methanolic extract of Cocculus hirsutus (L.). International Journal of Pharmacy Science and Research 2014 5:1957–1962.

Banagar AV, Shivakumar B, Jayaveera KN (2013) Effect of Xylia dolabriformis leaves extract on high fructose diet induced C57BL/6J ob/ob diabetic mice. International Journal of Pharmacy Science and Research 4(10):4032-45.

Banu N, Alam N, Islam MN, Islam S, Sakib SA, Hanif NB, Chowdhury MR, Tareq AM, Chowdhury KH, Jahan S, Azad A, Emran TB, Simal-Gandara J (2020) Insightful Valorization on Biological Activities of Pani Heloch Leaves through Experimental and Computer-Aided Mechanisms. Molecules 25 (21): 5153.

Barua N, Aziz MAI, Tareq AM, Sayeed MA, Alam N, Alam N, Uddin MA, Lyzu C, Emran TB (2020) In vivo and in vitro evaluation of pharmacological activities of Adenia trilobata (Roxb.). Biochemistry and Biophysics Reports 23: Article ID: 100772. DOI: 10.1016/j.bbrep.2020.100772.

Bauer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45: 493-496.

Biswas FB, Roy TG, Rahman MA, Emran TB (2014) An in vitro antibacterial and antifungal effects of cadmium (II) complexes of hexamethyl tetra-azacyclotetradecadiene and isomers of its saturated analogue. Asian Pacific Journal of Tropical Medicine 7 (Suppl. 2): S534-S539

Boucher HW, Talbot GH, Bradley JS (2009) Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clinical Infectious Diseases 48(1): 1–12.

Bristy TA, Barua N, Tareq AM, Sakib SA, Etu ST, Chowdhury KH, Jyoti MA, Aziz MAI, Reza ASMA, Caiazzo E, Romano B, Tareq SM, Emran TB, Capasso R (2020) Deciphering the pharmacological properties of methanol extract of Psychotria calocarpa leaves through in vivo, in vitro and in silico approaches. Pharmaceuticals 13 (8): 183.

Cheruvanky H (2004) A history of pathology and laboratory medicine at Baylor University Medical Center. United States Pathology 6(1): 733-741.

Cowan MM (1999) Plant products as antimicrobial agents. Clinical Microbiology Reviews 12(4): 564–582.

Doss A, Dhanabalan R (2008) Anti-hyperglycaemic and Insulin Release Effects of Coccinia grandis (L.) Voigt Leaves in Normal and Alloxan Diabetic Rats. Ethnobotanical Leaflets 12: 1172-1175.

Dutta T, Paul A, Majumder M, Sultan RA, Emran TB (2019) Pharmacological evidence for the use of Cissus assamica as a medicinal plant in the management of pain and pyrexia. Biochemistry and Biophysics Reports 21: 1-8. Article ID: 100715. DOI: 10.1016/j.bbrep.2019.100715.

Elisha IL, Dzoyem JP, Botha FS, Eloff JN (2016) The efficacy and safety of nine South African medicinal plants in controlling Bacillus anthracis Sterne vaccine strain. BMC Complementary Alternative Medicine 16: 5.

Emran TB, Rahman MA, Uddin MMN, Dash R, Hossen MF, Mohiuddin M, Alam MR (2015) Molecular docking and inhibition studies on the interactions of Bacopa monnieri’s potent phytochemicals against Staphylococcus aureus. DARU Journal of Pharmaceutical Sciences 23: 26.

Ghani A (2003) Asiatic Society of Bangladesh. (2nd Ed.): 138

Harborne JB (1973) Phytochemical methods: A guide to modern techniques of plant analysis. Chapman and Hall Ltd, London: 279.

Harizal SN, Mansor SM, Hasnan J, Tharakan JKJ, Abdullah J (2010) Acute toxicity study of the standardized methanolic extract of Mitragyna speciosa Korth in Rodent. Journal of Ethnopharmacology 131 (2): 404-409.

Jahan I, Tona MR, Sharmin S, Sayeed MA, Tania FZ, Paul A, Chy MNU, Rakib A, Emran TB, Simal-Gandara J (2020) GC-MS phytochemical profiling, pharmacological properties and in-silico studies of Chukrasia velutina leaves: A novel source for bioactive agents. Molecules 25(15): 3536. DOI: 10.3390/molecules25153536.

Kannur DM, Hukkeri VI, Akki KS (2006) Antidiabetic activity of Caesalpinia bonducella seed extracts in rats. Fitoterapia 77: 546-549.

Kishore G, Siva G, Sindhu ES (2011) In Vitro Anti-Inflammatory and Anti-Arthritic Activity of Leaves of Physalis Angulata L. Industrial Journal of Pharmacy and Industrial Research 1:211–213.

Kumar ABP, Sharma DEA., Varshney CP, Rao CV (2013) Antidiabetogenic and antioxidant effects of Caralluma attenuata extract on streptozotocin induced diabetes in rats. Journal of Pharmacy Research 7: 257-262.

Li YM, Li ZY, Ye M (2008) The chemical compositions and their bioactivities in the different parts of Eupatorium adenophorum Spreng. Journal of Yunnan Agricultural University 23: 42–46.

Lipsky PE (2008) In: Harrison’s principles of internal medicine. (17th Ed.). Mc. Hill Company, New York, 2: 2083.

Mizushima Y, Kobayashi M (1968) Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. Journal of Pharmacy and Pharmacology 20:169 -173.

Mohan SM, Pandey B (2016) Antimicrobial activity of Oxalis corniculata Linn. International Journal of Science and Research 5(7): 575-578.

Muhit MA, Tareq SM, Apu AS, Basak D, Islam MS (2010) Isolation and identification of compounds from the leaf extract of Dillenia indica Linn. Bangladesh Pharmaceutical Journal 13:49–53.

O'Brien T, Nguyen TT, Zimmerman BR (1998) Hyperlipidemia and diabetes mellitus. Mayo Clinic Proceedings 73(10): 969–976.

Patwardhan B, Hopper B (1992) Ayurvedic and future drug development. Journal of Alternative and Complementary Medicine 19: 9-10.

Rahman MA, Emran TB, Islam MS (2013) Antioxidative, antimicrobial and cytotoxic effects of the phenolics of Leea indica leaf extract. Saudi Journal of Biological Sciences 20: 213-225.

Rakib A, Ahmed S, Islam MA, Uddin MMN, Paul A, Chy MNU, Emran TB, Seidel V (2020) Pharmacological studies on the antinociceptive, anxiolytic and antidepressant activity of Tinospora crispa. Phytotherapy Research 34(11):2978-2984.

Romero CD, Chopin SF, Buck G, Martinez E, Garcia M, Bixby L (2005) Antibacterial properties of common herbal remedies of the southwest. Journal of Ethnopharmacology 99(2): 253–257.

Shifah F, Tareq AM, Sayeed MA, Islam MN, Emran TB, Ullah MA, Mukit MA and Ullah M (2020) Antidiarrheal, cytotoxic and thrombolytic activities of methanolic extract of Hedychium coccineum leaves. Journal of Advanced Biotechnology and Experimental Therapeutics 3(1): 77-83.

Silman AJ, Pearson JE (2002) Epidemiology and genetics of rheumatoid arthritis. Arthritis Research 4 (3): 265-272.

Sofowara A (1993) Medicinal plants and traditional medicine in Africa. (2nd Edition). Spectrum Books Limited, sunshine House, Ibadan, Nigeria: Pp.191-289.

Soto C, Muriel P, Reyes JL (1994) Pancreatic lipid peroxidation in alloxan-induced diabetes mellitus. Archives of Medical Research 25(4): 377-380.

Subramoniam A, Madhavachandran V, Gangaprasad A (2013) Review Article: Medicinal plants in the treatment of arthritis. Annals of Phytomedicine 2(1): 3-36.

Tareq AM, Farhad S, Uddin ABMN, Hoque M, Nasrin MS, Uddin MMR, Hasan M,Sultana A,Munira MS, Lyzu C, Hossen SMM, Reza ASMA, Emran TB (2020) Chemical profiles, pharmacological properties, and in silico studies provide new insights on Cycas pectinata. Heliyon 6(6): e04061.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H (2011) Phytochemical Screening and Extraction: A Review. Internationale Pharmaceutica Sciencia 1:98- 106.

Trease GE, Evans WC (1989) Pharmacognosy. (11th edn.). Brailliar Tiridel and Macmillian Publishers, London, UK.

Uddin MZ, Paul A, Rakib A, Sami SA, Mahmud S, Rana MS, Hossain S, Tareq AM, Dutta M, Emran TB and Simal-Gandara J (2021) Chemical Profiles and Pharmacological Properties with In Silico Studies on Elatostema papillosum Wedd. Molecules 26(4): 809. DOI: 10.3390/molecules26040809.

Uddin MZ, Rana MS, Hossain S, Dutta E, Ferdous S, Dutta M, Emran TB (2019) In vivo neuroprotective, antinociceptive, anti-inflammatory potential in Swiss albino mice and in vitro antioxidant and clot lysis activities of fractionated Holigarna longifolia Roxb. bark extract. Journal of Complementary and Integrative Medicine 17(2): 1-9.

Uddin SN (2002) Bangladesh National Herbarium: 541, 604.

Yan QS, Yang J, Li HM (2006) Advances in the studies on the chemical components and bioactivity of Eupatorium adenophorum Spreng as an intruding species. Journal of Beijing Normal University 42: 70–73.

Yesmin S, Paul A, Naz T, Rahman ABMA, Akhter SF, Wahed MII, Emran TB, Siddiqui SA (2020) Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba). Clinical Phytoscience 6:59.