Potential effect of fruit and flower extracts of Arbutus unedo L. on Tetrahymena pyriformis exposed to a cobalt-60 source

Fatine Belfekih; Ahmed Moussaif; Mohammed El Mzibri; Adnane Moutaouakkil; Laila Benbacer; Rachid Bengueddour; Abdelghani Iddar

doi:10.18006/2024.12(2).237.247

Authors

Fatine Belfekih Biotechnology and Engineering of Biomolecules Unit, National Centre for Nuclear Energy, Science and Technology (CNESTEN), Rabat, Morocco
Ahmed Moussaif Biotechnology and Engineering of Biomolecules Unit, National Centre for Nuclear Energy, Science and Technology (CNESTEN), Rabat, Morocco https://orcid.org/0000-0001-8444-0537
Mohammed El Mzibri Biotechnology and Engineering of Biomolecules Unit, National Centre for Nuclear Energy, Science and Technology (CNESTEN), Rabat, Morocco https://orcid.org/0000-0002-3148-1527
Adnane Moutaouakkil Biotechnology and Engineering of Biomolecules Unit, National Centre for Nuclear Energy, Science and Technology (CNESTEN), Rabat, Morocco https://orcid.org/0000-0002-1762-881X
Laila Benbacer Biomedical Research Unit, National Center for Energy, Sciences and Nuclear Techniques (CNESTEN), BP. 1382 RP, 10001 Rabat, Morocco https://orcid.org/0000-0002-4200-6158
Rachid Bengueddour Laboratory of Natural Resources and Sustainable Development, Biology Department, Faculty of Sciences, Ibn Tofail University, PB 133-14050, Kenitra, Morocco
Abdelghani Iddar Biotechnology and Engineering of Biomolecules Unit, National Centre for Nuclear Energy, Science and Technology (CNESTEN), Rabat, Morocco https://orcid.org/0000-0001-6111-0607

DOI:

https://doi.org/10.18006/2024.12(2).237.247

Keywords:

Arbutus unedo, Cobalt-60 source, Gamma radiation, Tetrahymena pyriformis, Aqueous extract, Ethanolic extract, Antioxidant

Abstract

Exposure of Tetrahymena pyriformis cultures to cobalt-60 for 72 h significantly impacted the cells' growth, appearance, and physiology. This study aims to investigate the protective effects of Arbutus unedo L flowers and fruit extracts on T. pyriformis against gamma radiation. Initially, aqueous and 50% ethanolic extracts of the fruits and flowers were prepared, and their cytotoxicity on the ciliate was evaluated. The irradiated ciliate's cellular viability and morphological aspect improved when a non-toxic concentration of 25 µg/mL was added to the growth medium. The addition of extracts restored glyceraldehyde-3-phosphate dehydrogenase and succinate dehydrogenase activities to their initial levels, similar to non-irradiated cells. In addition, the extracts reduced oxidative stress markers, such as lipid peroxidation, and decreased the activities of antioxidant defence enzymes, catalase, and superoxide dismutase. This may be attributed to the antioxidant properties of the extracts. Results of this study revealed that the flower extracts exhibited better protective effects than the fruit extracts, with superior antioxidant activity in the in-vitro DPPH scavenging assay. These results suggest that A. unedo flower extracts may have potential as exogenous radioprotective agents.

Author Biographies

Fatine Belfekih, Biotechnology and Engineering of Biomolecules Unit, National Centre for Nuclear Energy, Science and Technology (CNESTEN), Rabat, Morocco

Laboratory of Natural Resources and Sustainable Development, Biology Department, Faculty of Sciences, Ibn Tofail University, PB 133-14050, Kenitra, Morocco

Mohammed El Mzibri, Biotechnology and Engineering of Biomolecules Unit, National Centre for Nuclear Energy, Science and Technology (CNESTEN), Rabat, Morocco

Biomedical Research Unit, National Center for Energy, Sciences and Nuclear Techniques (CNESTEN), BP. 1382 RP, 10001 Rabat, Morocco

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