Cytotoxic Study of Zinc Oxide Nanoparticles on Cervical Cancer Cell Line

Harshyini Maheswaran; Sinouvassane Djearamane; Anto Cordelia Tanislaus Antony Dhanapal; Wong Ling Shing

doi:10.18006/2023.11(5).815.821

Authors

Harshyini Maheswaran Department of Biomedical Sciences, Faculty of Science, UniversitiTunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia
Sinouvassane Djearamane Department of Biomedical Sciences, Faculty of Science, UniversitiTunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia
Anto Cordelia Tanislaus Antony Dhanapal Department of Chemical Science, Faculty of Science, UniversitiTunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia
Wong Ling Shing Faculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia

DOI:

https://doi.org/10.18006/2023.11(5).815.821

Keywords:

Zinc oxide nanoparticles, Chemical synthesis, HeLa cell, FESEM, EDX, MTT assay

Abstract

The advancement of nanomedicine drugs as an outcome of nanotechnology offers tremendous potential to enhance cancer-fighting tactics. Scientists have begun studying the role of NPs in immunotherapy, an area that is particularly beneficial in treating malignancies. Conventional treatment of cancer uses medications known as chemotherapy that frequently cause adverse effects on healthy tissues. Zinc is a vital micronutrient for the well-being of humans; therefore, nanomaterials such as zinc oxide nanoparticles (ZnO NPs) are progressively appealing as cutting-edge medical agents with implementations like anticancer properties. A bottom-up approach was utilized to chemically produce the ZnO NPs, which were characterized using Field Emission Scanning Electron Microscope (FESEM) and Energy Dispersive X-ray analysis (EDX). MTT assays have been carried out to study the cell viability percentage against multiple ZnO NPs concentrations and durations. The white ZnO NPs displayed a diverse morphology within the nanoscale range, featuring rod and spherical shapes. This synthesis was confirmed through EDX, which revealed distinct peaks corresponding to zinc and oxygen, affirming the formation of pure ZnO NPs. MTT assay data showed that ZnO NPs had a dose and time-dependent cytotoxicity against HeLa cells. This observation suggests that the ZnO NPs possess the potential to combat cancer and may hold promise for applications in biomedical research, particularly in the development of anticancer drugs.

Author Biography

Sinouvassane Djearamane, Department of Biomedical Sciences, Faculty of Science, UniversitiTunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia

Biomedical Research Unit and Lab Animal Research Centre, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 602 105, India

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