Differentiation Effect of Two Alkaloid Fractions from Vietnamese Lycopodiaceae on Mouse Neural Stem Cells

Hanh-Nguyen Thi Tran; Huyen-Trang Thi  Nguyen; Dinh Hoang  Nguyen; Thuy Thi  La; Kim-Thanh Thi  Nguyen; Tien Dat  Nguyen; Quang Huy  Nguyen; Lai Thanh  Nguyen; My Hanh Thi Hoang

doi:10.18006/2022.10(1).64.72

Authors

Hanh-Nguyen Thi Tran Faculty of Biology, University of Science, Vietnam National University, Hanoi (VNU), Hanoi 10000, Vietnam
Huyen-Trang Thi Nguyen Faculty of Biology, University of Science, Vietnam National University, Hanoi (VNU), Hanoi 10000, Vietnam
Dinh Hoang Nguyen Faculty of Biology, University of Science, Vietnam National University, Hanoi (VNU), Hanoi 10000, Vietnam
Thuy Thi La Faculty of Biology, University of Science, Vietnam National University, Hanoi (VNU), Hanoi 10000, Vietnam
Kim-Thanh Thi Nguyen Faculty of Biology, University of Science, Vietnam National University, Hanoi (VNU), Hanoi 10000, Vietnam
Tien Dat Nguyen Center for Research and Technology Transfer, Vietnam Academy of Science and Technology, Hanoi 10000, Vietnam
Quang Huy Nguyen National Key Laboratory of Enzyme and Protein Technology, University of Science, Vietnam National University, Hanoi (VNU), Hanoi 10000, Vietnam
Lai Thanh Nguyen Faculty of Biology, University of Science, Vietnam National University, Hanoi (VNU), Hanoi 10000, Vietnam
My Hanh Thi Hoang Faculty of Biology, University of Science, Vietnam National University, Hanoi (VNU), Hanoi 10000, Vietnam

DOI:

https://doi.org/10.18006/2022.10(1).64.72

Keywords:

Lycopodium clavatum, Huperzia serrata, Lycopodium Alkaloid, Neuronal Disease, Mouse Neural Stem Cell

Abstract

Various Lycopodium alkaloids have been studied for their various biological activities including anti-inflammatory, antioxidant, immunomodulatory, and neuroprotective activities. Moreover, these alkaloid compounds have high potential in the treatment of neuron degenerative disease. This study has been carried out to test the effect of Huperzia serrata (Thunb.) Trevis, and Lycopodium clavatum L alkaloid fractions on the mouse neural stem cells (NSCs). Firstly, the alkaloid fractions were used to verify its toxicity on NSCs. The multiple concentrations of alkaloid fractions from H. serrata (0.044; 0.088; 0.175; 0.35; 0.7; 1.4 mg/ml) and L. clavatum (0.031; 0.063; 0.125; 0.25; 0.50; 1.0; 2.0 mg/ml) have been used for the treatment of NSCs at period of 48h incubation. Results of the study suggested that the IC₅₀ value of H. serrata and L. clavatum was 0.56 mg/ml and 0.50 mg/ml, respectively. Then, the NSCs were differentiated in the presence of 5 and 10 µg/ml of alkaloid fraction from H. serrata; 0.625 and 1.25 µg/ml of alkaloid fraction from L. clavatum for 6 days. Here, we observed the primary NSCs treated with alkaloid fraction extract from H. serrata showed the increased gene expression level of early neuron TUBB3 and neuron-specific cytoskeleton MAP2. On the other hand, the L. clavatum alkaloid fraction increased the expression of neural stem cell marker genes (Nestin and PAX6) and decreased neuron marker genes. In conclusion, these results established that alkaloid fraction from H. serrata promoted differentiation of the mouse NSCs to neuron cells, and L. clavatum extract had a capacity for stemness maintenance.

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