Current and emerging molecular technologies for the diagnosis of plant diseases –  An overview

Mohammad Malek Faizal Azizi; Noor Hani  Mardhiah; Han Yih Lau

doi:10.18006/2022.10(2).294.305

Authors

Mohammad Malek Faizal Azizi 1Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Malaysia
Noor Hani Mardhiah Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selango
Han Yih Lau Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Malaysia

DOI:

https://doi.org/10.18006/2022.10(2).294.305

Keywords:

ELISA, PCR, Real-time PCR, LAMP, Biosensor, NGS

Abstract

Plant diseases caused by numerous pathogens such as bacteria, viruses, and fungi are responsible for substantial economic losses in the agricultural industry worldwide. Specific, sensitive, and efficient diagnostic tools have been developed worldwide to mitigate and prevent the pathogenic threat. The diagnostic tools have revolutionized from classical methods to more advanced molecular diagnostic approaches such as enzyme-linked immunosorbent assay (ELISA), conventional polymerase chain reaction (PCR), real-time PCR, loop-mediated isothermal amplification (LAMP), biosensor, and next-generation sequencing (NGS). Hence, this review describes the current and emerging molecular diagnostic tools to distinguish and identify pathogens in crops.

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