Isolation and determination of Vibrio spp. pathogen from Sciaenops ocellatus  suffering from hemorrhagic disease under cage culture in Vietnam

Nguyen Quang Linh; Pham Thi Hai  Yen; Nguyen Duy Quynh  Tram

doi:10.18006/2022.10(2).405.415

Authors

Nguyen Quang Linh Hue University, 03 Le Loi, Hue 49000, Vietnam https://orcid.org/0000-0002-8424-1148
Pham Thi Hai Yen University of Agriculture and Forestry, Hue University, Hue 49000, Vietnam https://orcid.org/0000-0001-8852-5044
Nguyen Duy Quynh Tram University of Agriculture and Forestry, Hue University, Hue 49000, Vietnam https://orcid.org/0000-0001-5362-0391

DOI:

https://doi.org/10.18006/2022.10(2).405.415

Keywords:

Antibiotic, Cage culture, Hemorrhagic disease, Red drum, Sciaenops ocellatus, Vibrio

Abstract

This study was carried out to isolate and determine the Vibrio spp. from the Red drum fish (Sciaenops ocellatus) suffering from the hemorrhagic disease in Vietnam. In this study, 18 strains of Vibrio bacteria were identified from 27 samples of Red drum fish. The isolated bacterial strains were identified with the 16S rRNA sequencing method and checked for morphological, physiological, and biochemical characteristics by using the API 20E KIT. Results of the study revealed the presence of twelve strains of V. alginolyticus, three strains of V. fluvialis, and three strains of V. orientalis. All Vibrio strains have gene similarities with those on the Genbank ranging from 98.05 to 100%. The biochemical characteristics of these 18 isolates were similar and these are susceptible to tetracycline and doxycycline and entirely resistant to ampicillin, amoxicillin, and erythromycin.

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