Development of the bacterial consortia for the degradation of benzo[a]pyrene,  pyrene from hydrocarbons waste

Beema Kumari; Ram Chandra

doi:10.18006/2023.11(4).671.682

Authors

Beema Kumari Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Lucknow-226025, Uttar Pradesh-226025, India https://orcid.org/0000-0003-4343-1065
Ram Chandra Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Lucknow-226025, Uttar Pradesh-226025, India https://orcid.org/0000-0001-7070-6074

DOI:

https://doi.org/10.18006/2023.11(4).671.682

Keywords:

Benzo[a]pyrene, Pyrene, Consortia, PAHs, Bioremadiation

Abstract

The environment is heavily populated with polycyclic aromatic hydrocarbons (PAHs), which are dangerous to human health. Degradation and cleaning of PAH chemicals from water and soil regions are crucial due to their chemical and biological impacts and persistent nature. In this study, we found that a very efficient bacterial consortium A-LOBP-19A+LOP-9 (99.62%) for benzo[a]pyrene up to 1000ppm and B-LOP-9 +GWP-2 (93.8%) for pyrene up to 2000ppm concentration degradation and it was done in MSM medium with isolated bacterial strains and incubated at 37° C for 50 days and 30 days respectively. This consortium consisting of the Mycobacterium vaanbaalenii GWP-2 (ON715011), Staphylococcus aureus LOP-9(ON715121), and Stutzerimonas stutzeri (LOBP-19A) OP389146, and these have capabilities of mentioned PAHs. The HPLC analysis suggested that both benzo[a]pyrene and pyrene degraded through peaks by both consortia. Degraded metabolites were identified by GC-MS and reported the presence of Phthalic acid, Naphthalene, 1,4-benzodicarboxylic acid, Butoxyacetic acid, Benzeneacetic acid and benzo [a]pyrene-1,6-dione. Thus, the study demonstrated efficient bacterial community enhancement for PAHs (benzo[a]pyrene, pyrene) decomposition, and these can be further explored for the cleanup of hydrocarbons pollution.

Author Biography

Beema Kumari, Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Lucknow-226025, Uttar Pradesh-226025, India

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