A COMPARATIVE PHARMACOGNOSTIC STUDY OF THE TWO Orthoshipon aristatus (BLUME) MIQ. VARIETIES
DOI:
https://doi.org/10.18006/2021.9(Spl-2-ICOPMES_2020).S228.S233Keywords:
Orthosiphon aristatus, Purple variety, White variety, Crude drugs characteristicAbstract
The use of a plant as an ingredient in traditional medicine requires scientific evidence to determine its properties. Cat's whiskers (Orthosiphon aristatus) is one of the widely used traditional medicinal plants in various Asian and European countries. This study aimed to determine the pharmacognostic properties of purple and white varieties of O. aristatus. Aqueous and ethanolic extract of the stem and leaves of purple and white varieties of O. aristatus was prepared and investigated for the presence of active ingredients. The AlCl3 colorimetric method was used for the estimation of flavones and flavonols. The level of flavonoid was reported 13.06 ± 0.13 mg QE / 1g and 6.17 ± 0.049 mg QE / 1g for the leaves and stem extracts of purple varieties respectively while this value was reported 9.76 ± 0.15 mg QE / 1g and 3.79 ± 0.03 mg QE / 1g for the white variety. From the results of the study, it can be concluded that the purple variety has a significantly higher amount of flavonoid then the white variety.
References
Abraika OSS, Atangwho IJ, Sadikun A, Asmawi MZ, Hussain EA (2012) In vitro activity-guided vasodilatory effect of Orthosiphon stamineus leaves. Journal of Experimental and Integrative Medicine 2 (3): 255–261. https://doi.org/10.5455/jeim.080612.or.033.
Adawiyah R, Nur B, Che A, Tantowi A, Fong S, Suhaila L (2018) Java Tea ( Orthosiphon stamineus ) is protected against osteoarthritis by mitigating inflammation and cartilage degradation: a preclinical study. Inflammopharmacology.https://doi.org/10.1007/s10787-017-0432-2.
Ahmad AR, Juwita J, Ratulangi SAD (2015) Determination of Total Phenolic and Flavonoid Levels of Methanol Extract of Patikala (Etlingera elatior (Jack) R.M.SM) Fruit and Leaf Extract. Pharmaceutical Sciences and Research 2 (1): 1-10. DOI: 10.7454 / psr.v2i1.3481.
Alshawsh M, Abdulla M, Ismail S, Amin Z, Qader S, A Hadi H, Harmal N (2012) Free Radical Scavenging, Antimicrobial and Immunomodulatory Activities of Orthosiphon stamineus.Molecules 17:5385–5395. https://doi.org/10.3390/molecules17055385.
Ameer OZ, Salman IM, Asmawi MZ, Ibraheem ZO, Yam MF (2012) Orthosiphon stamineus: Traditional Uses, Phytochemistry, Pharmacology, and Toxicology. Journal of Medicinal Food 15 (8): 678–690. https://doi.org/10.1089/jmf.2011.1973.
Anonymous (2017) 2nd edition of the 2017 Indonesian Herbal Pharmacopeia.Ministry of Health of the Republic of Indonesia, Jakarta.
Azizah DN, Kumolowati E, Faramayuda F (2014) Determination of Flavonoid Levels using AlCl3 Method in Methanol Extract of Cocoa Fruit Peel (Theobroma cacao L.). Kartika Jurnal Ilmiah Farmasi 2(2):45-49.
Cai X, Xiao C, Xue H, Xiong H, Hang Y, Xu J, Lu Y (2018) A Comparative Study of The Antioxidant and Intestinal Protective Effects of Extracts from Different Parts of Java Tea (Orthosiphon stamineus). Food Science and Nutrition 6(3): 579–584.DOI: 10.1002 / fsn3.584.
Chang CC, Yang MH, Wen HM, Chern JC (2002) Estimation of total flavonoid content in propolis by two complementary colometric methods. Journal of Food and Drug Analysis 10(3):178-182.
Chen C (1989) Screening of Taiwanese crude drugs for antibacterial activity against Streptococcus. Journal of Ethnopharmacology 27(3):285-95. DOI: 10.1016/0378-8741(89)90003-2. PMID: 2615434.
Faramayuda F, Mariani TS, Elfahmi, Sukrasno (2020) Short Communication : Callus induction in purple and white-purple varieties of Orthosiphon aristatus ( Blume ) Miq. Biodiversitas 21(10), 4967–4972.
Faramayuda F, Mariani TS, Elfahmi, Sukrasno (2021a) Phytochemical analysis of callus two varieties Orthosiphon aristatus (blume) miq on murashige and Skoog media. a strategic step of secondary production. International Journal of Applied Pharmaceutics 13(2): 71-77.
Faramayuda F, Mariani TS, Elfahmi E, Sukrasno S (2021b) Research Potential of Orthosiphon aristatus BlumeMiq as Antiviral : A Review, Tropical Journal of Natural Product 5: 410–419.
Faramayuda F, Mariani TS, Elfahmi, Sukrasno. (2021c). Identification of secondary metabolites from callus Orthosiphon aristatus (Blume) Miq by thin layer chromatography. Sarhad Journal of Agriculture, 37(3): 1081-1088.
Febjislami S, Kurniawati A, Melati M, Wahyu Y ( 2019) Morphological characters, flowering and seed germination of the Indonesian medicinal plant Orthosiphon aristatus. Biodiversitas 20:328–337.
Fitria V, Arifin RF, Kurniasih N (2017) Activity test of pohpohan leaf extract gel (Pilea trinervia W.) against burns healing in rabbits (Oryctolagus cuniculus). Kartika : Jurnal Ilmiah Farmasi 5(2):75. doi:10.26874/kjif.v5i2.120.
George A, Chinnappan S, Choudhary Y, Choudhary VK, Bommu P, Wong HJ (2015) Effects of a Proprietary Standardized Orthosiphon Stamineus Ethanolic Leaf Extract on Enhancing Memory in Sprague Dawley Rats Possibly via Blockade of Adenosine A 2A Receptors. Evidence-Based Complementary and Alternative Medicine2015:375837. DOI: 10.1155/2015/375837. Epub 2015 Nov 8. PMID: 26649059; PMCID: PMC4655036.
Halim NH, Pauzi N, Hamil SHR, Shafaei A, Ismail Z, Mohd KS (2017) Standardization of Orthosiphon stamineus Raw Materials and Extracts for Anti-Uterine Fibroids. International Journal of Pharmacognosy and Phytochemical Research 9 (4): 512–515.
Hermawan DS, Lukmayani Y, Dasuki UA (2016) Identification of Flavonoid Compounds in Extracts and Fractions Derived from Berenuk Fruit (Crescentia cujete L.). Pharmacy Proceedings 2(2):253-259.
Ho C, Noryati I, Sulaiman S, Rosma A (2010) In vitro antibacterial and antioxidant activities of Orthosiphon stamineus Benth. extracts against food-borne bacteria. Food Chemistry 122 (4):1168–1172. https://doi.org/10.1016/j.foodchem.2010.03.110.
Hossain MA, Ismail Z, Rahman A, Kang SC (2007) Chemical composition and anti-fungal properties of the essential oils and crude extracts of Orthosiphon stamineus Benth. Industrial Crops and Products 7:328–334.https://doi.org/10.1016/j.indcrop.2007.11.008.
Kar B, Choo M, Kundap UP, Kumari Y, Hue S, Othman I, Shaikh MF (2018) Orthosiphon stamineus Leaf Extract Affects TNF-α and Seizures in a Zebrafish Model. Frontiers in Pharmacology 9 (February), 1–11. https://doi.org/10.3389/fphar.2018.00139.
Lai Keng C, Poay Siong L (2006) Morphological Similarities and Differences between the Two Varieties of O. aristatus (Orthosiphon stamineus Benth.) Grown in Malaysia. International Journal of Botany. 2.https://doi.org/10.3923/ijb.2006.1.6.
Lee W (2004) micropropagation and cell culture of misai kucing (Orthosiphon Stamineus Benth.) and detection of rosmarinic acid in the in vitro cultures, Thesis.
Mohamed E, Mohamed AJ, Abdullah M, Sadikun A, Saad Ebrika O,
Yam M (2011) Antihyperglycemic Effect of Orthosiphon Stamineus Benth Leaves Extract and Its Bioassay-Guided Fractions. Molecules 16: 3787–3801. https://doi.org/10.3390/molecules16053787.
Nuari S, Anam S, Khumaidi A (2017) Isolation and identification of flavonoid compounds in thick extract of bitter melon fruit. Jurnal Farmasi Galenika 2(2):118-125.
Olah NK, Hanganu D, Bodoki E, Oprean R, Toma C, Morgovan C, Gocan S (2017) Characterization of Orthosiphon stamineus Benth extracts by reversed-phase thin-layer chromatographic methods. Studia Universitatis Babes-Bolyai Chemia 62(3): 9–18.
Pauzi N, Mohd KS, Hidayah N, Halim A (2018) Orthosiphon stamineus Extracts Inhibits Proliferation and Induces Apoptosis in Uterine Fibroid Cells. Asian Pacific Journal of Cancer Prevention 19:2737–2744. https://doi.org/10.22034/APJCP.2018.19.10.2737.
Ripim M, Fazil N, Ibrahim K, Bahtiar A, Wai C, Ibrahim N, Nor M (2018) Antiviral Properties of Orthosiphon stamineus Aqueous Extract in Herpes Simplex Virus Type 1 Infected Cells. Malaysians Science 47 (8):1725-1730.
Tezuka Y, Stampoulis P, Banskota AH, Awale S, Tran KQ, Saiki I, Kadota S (2000) Constituents of the Vietnamese medicinal plant Orthosiphon stamineus. Chemical and Pharmaceutical Bulletin 48(11):1711-9. DOI: 10.1248/cpb.48.1711.
Utami YP, Taebe B, Fatmawati (2016) Standardization of Specific and Non-Specific Parameters of Ethanol Extract of Mulberry (Morus alba L.) Leaves from Soppeng Regency, South Sulawesi Province. Journal of Pharmaceutical and Medicinal Sciences1(2):48-52.
Xue HQ, Cai X, Xiong HH, Hang YQ, Xiao CF, Lu YH (2016) Main Nutrients and Antioxidant Capacity of Orthosiphon stamineus in Different Parts. Acta Agriculturae Shanghai (in Chinese).2(3): 30–35. https://doi.org/10.15955/j.issn1000-3924.2016.03.06.
Yuniarto ARI, Susilawati E, Khairunnisa I, Juanda D, Setiawan F (2017) Antioxidant and Gastric Ulcer Healing Effect of Orthosiphon stamineus (Benth.) Leaves Extract In Aspirin-Induced Rats. Asian Journal of Pharmaceutical and Clinical Research 10 (2):2–4. DOI: http://dx.doi.org/10.22159/ajpcr.2017.v10i2.15873.
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