ASSESSMENT OF PROBIOTIC PROPERTIES AND SAFETY OF LACTIC ACID BACTERIA ISOLATED FROM SOUTH SULAWESI ETHNIC CHEESE

Nana Juniarti Natsir  Djide; Nur Indah  Yanti; Nurul Suci  Pratiwi; Andi Ainun  Nuzulia; Rangga Meidianto  Asri

doi:10.18006/2021.9(Spl-2-ICOPMES_2020).S222.S227

Authors

Nana Juniarti Natsir Djide Department of Microbiology, Faculty of Pharmacy, Hasanuddin University, Jl. PerintisKemerdekaan No.10, Makassar, Sulawesi Selatan, 90245, Indonesia
Nur Indah Yanti Department of Pharmacology, Faculty of Pharmacy, Hasanuddin University, Jl. PerintisKemerdekaan No.10, Makassar, Sulawesi Selatan, 90245, Indonesia
Nurul Suci Pratiwi Department of Microbiology, Faculty of Pharmacy, Hasanuddin University, Jl. PerintisKemerdekaan No.10, Makassar, Sulawesi Selatan, 90245, Indonesia
Andi Ainun Nuzulia Department of Microbiology, Faculty of Pharmacy, Hasanuddin University, Jl. PerintisKemerdekaan No.10, Makassar, Sulawesi Selatan, 90245, Indonesia
Rangga Meidianto Asri Department of Pharmaceutical Technology, Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan No.10, Makassar, Sulawesi Selatan, 90245, Indonesia

DOI:

https://doi.org/10.18006/2021.9(Spl-2-ICOPMES_2020).S222.S227

Keywords:

Lactic acid bacteria, Probiotic properties, Safety, South Sulawesi, Dangke

Abstract

Probiotic bacteria have been used widely as a functional food and health supplements. The functionality and safety of probiotics are the prerequisites given by WHO (World Health Organization) and FAO (Food Agricultural Organization) before utilizing probiotics. This study focuses on assessing probiotic properties and the safety of the lactic acid bacteria (LAB) isolated from dangke—traditional cheese of South Sulawesi. In the current study, the assessment of probiotic properties was carried by assessing its tolerance against low pH and bile salts. Safety assessments were divided into two assays viz., susceptibility testing and hemolytic activity. LAB from dangke demonstrated tolerance against low pH, bile salt and susceptibility against four types of antibiotics (ampicillin, cefotaxime, erythromycin, and tetracycline). Unfortunately, resistance towards gentamycin and an a-hemolytic activity was observed. This bacterium met the functional criteria from probiotics but failed to meet the safety criteria for probiotic safety.

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