Volume 6, Issue 6, December Issue - 2018, Pages:973-982
Authors: Pawan Shukla, Ramesha A. Reddy, Kangayam M. Ponnuvel, Gulab Khan Rohela, Aftab A Shabnam, S. S. Chauhan, M. K. Ghosh, Rakesh Kumar Mishra
Abstract: Low temperature/ cold stress is a major abiotic stress affecting mulberry plant growth and productivity in temperate climatic conditions. Mulberry leaf is a sole food source for the economically important insect, Bombyx mori. Cold stress severely damages buds and young leaves and affecting growth and leaf productivity. Therefore, it is required to identify genes which are responsive and impart cold tolerance and utilize them to develop improved mulberry varieties, which can withstand cold stress without compromising its leaf production. In the present study, candidate cold tolerant gene sequences of WRKY46, Spermidine synthase, early response to dehydration10 (ERD10), TIFY10, Cysteine proteinase Inhibitor (CPI), Cold regulated 413 plasma membrane protein 2 (COR413) were retrieved from the mulberry database through NCBI and bioinformatics tools. Comparative gene expression analysis was performed in temperate mulberry varieties, Gosherami, Leh 2 (saspole) and Gurez using tropical variety, G4 as a reference in cold stress condition. Results depicted that WRKY46, Spermidine synthase, ERD10, TIFY10, CPI, COR413 genes showed significant up-regulation under cold stress condition. Gurez genotype displayed significant up-regulation of these selected cold tolerant genes under both laboratory and field conditions compared to other mulberry genotypes.