AMINO ACID COMPOSITION AND ANTI-TYROSINASE ACTIVITY OF METABOLITES FROM EDIBLE Pleurotus SPECIES FOR THEIR NUTRITIONAL AND THERAPEUTIC POTENTIAL

Iyabo O.  Omomowo; Comfort O.  Bamigboye; Olawale I.  Omomowo; Olusola N.  Majolagbe; Adijat F.  Ogundola; Julius K.  Oloke

doi:10.18006/2021.9(6).837.850

Authors

Iyabo O. Omomowo Department of Pure and Applied Biology, LadokeAkintola University of Technology, P.M.B. 4000, Ogbomoso, Oyo State, Nigeria
Comfort O. Bamigboye Department of Pure and Applied Biology, LadokeAkintola University of Technology, P.M.B. 4000, Ogbomoso, Oyo State, Nigeria
Olawale I. Omomowo Department of Microbiology, University of Maiduguri, P.M.B. 1069 Maiduguri, Maiduguri, Borno State, Nigeria
Olusola N. Majolagbe Microbiology Programme, College of Agriculture, Engineering and Sciences, Bowen University Iwo, Osun State, Nigeria
Adijat F. Ogundola Department of Pure and Applied Biology, LadokeAkintola University of Technology, P.M.B. 4000, Ogbomoso, Oyo State, Nigeria
Julius K. Oloke Department of Pure and Applied Biology, LadokeAkintola University of Technology, P.M.B. 4000, Ogbomoso, Oyo State, Nigeria

DOI:

https://doi.org/10.18006/2021.9(6).837.850

Keywords:

Edible oyster mushroom, Health-promoting properties, Anti-tyrosinase inhibition, Amino acid quantity, Crude extract metabolite

Abstract

Mushrooms are being extensively researched due to their nutritional value and medicinal importance. The genus Pleurotus is the second most cultivated mushroom and is known for its high nutritional value, therapeutic properties, taste, flavor, as well as their application in biotechnology and environmental study. Also, tyrosinase is prevalent in most living organisms. The enzyme catalyzes the oxidation of monophenols to ortho-quinones in a two-step reaction process. This study was aimed to assess the amino acid composition and anti-tyrosinase activity of metabolites obtained from edible Pleurotus species. Assessment of the nutritional content and inhibitory studies of mushroom tyrosinase produced from four Pleurotus strains was carried out using high-performance liquid chromatography (RP-HPLC). The results of the study showed that seventeen different amino acids were identified in the crude and partially purified protein metabolites. Also, the crude extract metabolite had the highest quantity of amino acids than the partially purified. The highest and lowest amino acids value Glutamic acid (1343.26 µmol/mL) and valine (0.34 µmol/mL). The anti-tyrosinase inhibition studies of the four Pleurotus strains showed varying results from significantly inhibitory effects to slightly inhibitory effects on mushroom tyrosinase. The highest inhibition was 14.86% (Pleu-W), while the lowest inhibition was 51.42% (Plof-30) respectively. The high point of this study is that the Pleurotus species contains a significant number of amino acids and also, they possess good anti-tyrosinase activity. Therefore, these are a good source of nutritional and therapeutic metabolites and these can be explored further for their nutritional and medicinal importance to man.