Volume 8, Issue 1, February Issue - 2020, Pages:63-70
|Authors: Renjith Sebastian, M. Sravanthi, K. Ganesh, V. Umapathi, K. Narayanan, M. Priyanka, H.J. Dechamma, A. Sanyal, G.R. Reddy|
|Abstract: Macrophages represent a major class of innate immune cells mainly derived from peripheral blood monocytes. Monocytes migrate into extra-vascular space in response to wide range of stimuli and differentiate into macrophages. The differentiation of monocytes into macrophages involves morphological and functional changes through the co-ordinated regulation of numerous genes. The functional changes, especially changes in cytokine profile and arginine metabolism during differentiation of monocytes into macrophages is largely unknown. In this study, the difference in the expression of major cytokines and arginine metabolizing enzymes during in vitro differentiation of bovine monocytes to macrophages was investigated. Monocytes were obtained from the whole blood of indigenous cattle by immunomagnetic separation, and differentiated into monocyte-derived macrophages (MDM) using M-CSF. There was a significant down regulation of pro-inflammatory cytokines with concomitant up regulation of anti-inflammatory cytokines in the MDM as compared to primary monocytes. Further, the expression of Type I and II interferons was significantly down regulated in MDM during in vitro maturation. To understand the potential changes in arginine metabolism during in vitro maturation of monocytes to MDM, the expression of inducible nitric oxide synthase (iNOS) and arginase 1 (ARG1) was also studied. A significant up regulation of iNOS by 3-folds and down regulation of ARG1 by 2-folds in MDM suggested that the differentiation of monocytes into MDM is associated with induction of NO production from arginine.|
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