Volume 8, Issue 1, February Issue - 2020, Pages:30-34
|Authors: Shardulya Shukla, Yogesh Rajbhar, Ankita Sharma, Pushpender Bhardwaj, Vivek Kumar Tiwari, Harendra Tiwari, Manoj Kumar, Ashish Kumar|
|Abstract: Present study was conducted to study the effect of different auxins concentration on callus induction and root regeneration in Musa paradisiaca L. cv. Udhayam. MS media supplemented with 2, 4-D alone (2.00, 3.00, 4.00, 5.00, 6.00, mgl-1) was used for induction of callus whereas MS media was supplemented with nine combinations of IBA and NAA were used for regeneration of roots from induced callus. Result of study revealed that minimum time (24.41 days) of callus induction was observed in 2,4-D (4.00 mgl-1); whereas maximum time (39.42 days) was noted in control. Further, combination of IBA (2.50 mgl-1) and NAA, (2.00 mgl-1) also have significant effect on the root proliferation in banana shoots. Minimum days taken for root initiation (11.73 days) were found in IBA (2.50 mgl-1) and NAA (2.00 mgl-1) combination. Similarly, maximum number of roots (7.03) and maximum rooting percent (87.30%) per plantlet was recorded under the treatment of IBA (2.5mgl-1) and NAA (2.00 mgl-1). Therefore from the results of current study it can be concluded that 2,4-D with concentration of 4.00 mgl-1 has good response for callus induction whereas the combination of IBA (2.50 mgl-1) and NAA (2.00 mgl-1) was found better for regeneration of roots.|
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