Volume 7, Issue 6, December Issue - 2019, Pages:574-578
|Authors: Nausheen Saba Khanam, Asha Agarwal, Rafat Khan|
|Abstract: The aim of present study was to investigate the level of skin antioxidants and MDA in albino rats under stress of acute (1 day) and sub acute (30 days) artificial UVB exposure. Twenty healthy male albino rats (100-140 g) were taken for the present experiment. Rats were grouped into four sets (5 rats in each set). The Set A (Control set) and Set A1 (Control set) were not exposed to artificial UVB radiation while Set B was exposed to 0.44 J/cm2 of artificial UVB radiation continuously for 10 hours for 1 day and Set B1 was exposed to 0.014 J/cm2 of UVB radiation continuously for 20 min/day for 30 days. Results of study indicates a significant decrease in Catalase, SOD level and significant increase in MDA level in the skin of albino rats after acute and sub acute artificial UVB exposure. Present study also suggests decrease in SOD and Catalase content, while increase in MDA content in the skin of albino rats of Set B is more than the Set B1. Study concluded that the UVB radiation causes damage to the antioxidant system leads to decrease in Catalase and SOD level while induces the lipid peroxidation which increases MDA level in the skin of albino rat.|
Aebi H (1984) Catalase in vitro. Methods in Enzymology 105:121-126.
Anila L, Vijayalakshmi NR (2003) Antioxidant action flavonoids from Mangifera indica and Emblica officinalis in hypercholesterolemic rats. Food chemistry 83:569-574.
Apostolova MD, Nishimura N, Tohyama C (1995) Studies on the effects of oxidative stress due to UV-B irradiation. National Institute for environmental studies, Environmental agency 151-157.
Aricioglu A, Bozkurt M, Balabanli B, Kilinc M, Nazaroglu NK, Turkozkan N (2001) Changes in zinc levels and superoxide dismutase activities in the skin of acute, ultraviolet B-irradiated mice after treatment with Ginkgo biloba extract. Biological Trace Element Research 80:175-176.
Ashawat MS, Shailendra S, Swarnlata S (2007) Biochemical and Histopathological studies of herbal cream against UV radiation induced damage. Trends in Medical Research 2:135-141.
Ayala A, Munoz MF, Arguelles S (2014) Lipid peroxidation : Production Metabolism, and signaling mechanisms of malonyl dialdehyde and 4-hydroxy-2-nonenal. (Review Article). Oxidative Medicine and Cellular Longevity, Hindawi Publishing Corporation, Volume 2014, Article ID 360438, 31 pages. http://dx.doi.org/10.1155/2014/360438.
Bacci S, Romagnoli P, Streilein JW (1998) Reduction in number and morphologic alterations of langerhans cells after UVB radiation in vivo are accompanied by an influx of monocytoid cells into the epidermis. Journal of Investigative Dermatology 111 : 1134-1139.
Badescu L, Ciocoiu M, Petreus T, Neamtu M, Badescu M, Cotrutz CE (2012) Issues of epidermal senescence versus experimental Photo senescence on murine skin. Annals of the Romanian Society for Cell Biology XVII: 1:206-210.
Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods in Enzymology 52:302-310.
Chang C, Zheng R (2003) Effects of ultraviolet B on epidermal morphology, shedding, lipid peroxide, and antioxidant enzymes in cope’s rat snake (Elaphe taeniura). Journal of Photochemistry and Photobiology B : Biology. 72:79-85.
Clydesdale GJ, Dandie GW, Muller HK (2001) Ultraviolet light induced injury: Immunological and inflammatory effects. Immunology and Cell Biology 79:547-568.
Cui LL, Liang YY, Dong FJ, Ma L, Tu Y, Liu HY, Jiang J (2011) Structure of rat skin after application of electret characterized by DSC. Journal of Physics: Conferences series 301: 012027.
D’orazio J, Jarrett S, Amaro-Ortiz A, Scott T (2013) UV radiation and the skin. International Journal of Molecular Sciences 14:12222-12248.
Devasagayam TPA, Baloor KK, Ramasarma T (2003) Methods for estimating lipid peroxidation : An analysisof merits and demerits. Indian Journal of Biochemistry and Biophysics 40 : 300-308.
El-Shafey AAM, Seliem MME, El-Zawahry SAM, Shahen EMS, Mahmoud DEM (2015) Effect of honey bee on some antioxidant enzymes and lipid profile in hypercholesterolemic male albino rats. Zagazig University Medical Journal. 21:68-78.
Esterbauer H, Cheeseman KH (1990) Dermination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods in Enzymology 186:407-421.
Finney DJ (1971) Probit analysis, Cambridge University Press, Pp. 303.
Fisher RA, Yates (1963) Statistical tables for Biological, Agriculture and Medical Research. Longman VI edition, X+146 pp.
Gallagher RP, Lee TK, Bajdik CD, Borugian M (2010) Ultraviolet
George KS, Elyassaki W, Wu Q, Wu S (2012) The role of cholesterol in ultraviolet light B-induced apoptosis. Journal of Photochemistry and Photobiology 88:1191-1197.
Juzeniene A, Moan J (2012) Beneficial effects of UV radiation other than via vitamin D production. Dermato-Endocrinology 4:109-117.
Katiyar SK, Meleth S, Sharma SD (2008) Silymarin, a Flavonoid from milk thistle (Silybum marianum L.), inhibits UV-induced oxidative stress through targeting infiltrating CD 11b+ cells in mouse skin. Journal of Photochemistry and Photobiology 84:266-271.
Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the auto oxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 47:469-474.
Pai VV, Shukla P, Kikkeri NN (2014) Antioxidants in dermatology. Indian journal of Dermatology 5:210-214.
Pastore S, Korkina L (2010) Redox imbalance in T cell mediated skin diseases (Review article). Mediators of Inflammation. Volume 2010 article ID 861949, 9 Pages. Hindawi Publishing Corporation.
Svobodova A, Walterova D, Vostalova J (2006) Ultraviolet light induced alteration to the skin. Biomed Papers of the Medical Faculty of University Palacky Olomouc Czech Republic 150:25-38.