Volume 7, Issue 3, June Issue - 2019, Pages:329-334
|Authors: El-Masry KA, El-Fouly HA, Gabr SA|
|Abstract: The objective of present study was to determine the pattern and impact of pregnancy stage on the concentrations in serum estradiol-17β (E2-17β), testosterone hormones and immunoglobulin G (IgG). For this purpose, blood samples were collected from 16 crossbred, pregnant, lactating cows at the intervals of each two weeks. From these collected samples, serum hormone concentration was determined by Radioimmunoassay (125I-RIA) technique and IgG concentration was assayed by using the method involves antigen diffusing radially. Results of present study revealed that concentration of E2-17β is insignificant from 1st to the 4th month of gestation and tended to increase from the 4.5th month to the end of gestation. After parturition E2-17β returned to its normal level on 30th day of postpartum. Further, concentration of testosterone hormone start increasing (29.76 pg/ml) from the 4th month, after this it gradually increased (P <0.01) with the advances of gestation (185.40 pg/ml) up to the 9th month, thereafter testosterone level reached to the peak (197.80 pg/ml) at the end of pregnancy as compared to the average during non-pregnancy period. Concentration of IgG remained at basal level from the 1st to 6.5th month of gestation that averaged 23.65 g/l. Thereafter a sharpest increase in IgG level was occurred up to the 8.5th month that followed by a depression in the subsequent period until parturition. It can be concluded that concentrations of E2-17β and testosterone remained at low levels around the 4th – 5th month and progressively increased and showing a sharp rise in the 9.3 month of gestation. Moreover, the levels of IgG insignificantly changed until the 6.5th month of pregnancy and a sharpest rise occurred in the 8.5th month of gestation.|
Al- Anbaky KIH (2009) A study of serum steroid hormones concentration of pregnant cows. Iraqi Journal of Veterinary Medicine 33: 180 – 182.
Carolina L, Rodrigues MN, Favaron PO, Miglino MA (2014) Immunoglobulin transport during gestation in domestic animals and humans: A review. Open Journal of Animal Sciences 4:323-336.
Carlsen SM, Jacobsen G, Romundstad P (2006) Maternal testosterone levels during pregnancy are associated with offspring size at birth. European Journal of Endocrinology.155:365-370.
Elbanna IM, El-Asrag HA, Gamal MH (1988) An improved method for estradiol-17β radioimmunoassay. Isotope and Radiation Researches 20: 141-144.
El-Fouly HA, El- Masry KA, Gamal MH (1998a) Maternal body weight during trimester of pregnancy as an indicator of the development of male and female fetuses and their relationship with some steroid hormones and blood biochemical levels in Baladi cows. Zagazig Veterinary Journal 26: 56-68.
El-Fouly HA, El-Masry KA, Gamal MH (1998b) Effect of calves sex on some reproduction traits of Balady cows and growth performance of their Balady and crossbred calves. Zagazig Veterinary Journal 26: 69-78.
El-Masry KA, Yousef HM, Farghaly HA (1997) Hormonal pattern in pregnant Egyptian buffuloes in relation to their productivity. Buffalo Journal 2:147-156.
Emara SS (2015) Oxidative stress in relation to physiological and productivity changes in cattle. Ph.D. Thesis submitted to the Faculty of Agriculture, Ain Shams University.
Gitau R, Adams D, Fisk N, Glover V (2005) Fetal plasma testosterone correlated positively with cortisol. Archives of Disease in Childhood, Fetal and Neonatal Edition 90: 166-169.
Harichandan PP, Mohanty DN, Das S, Patra BK (2014)Hormonal and immunological studies in different trimester of pregnancy in cows. Indian Journal of Animal Reproduction 35: 42-44.
Herr M, Bosdedt H, Failing K (2011) Ig G and Ig M levels in dairy cows during the periparturient period. Theriogenology 15: 377-385.
Hirako M, Takahashi T, Takahashi H, Patel O, Domeki I (2003) Changes in plasma estrogen concentrations during the first trimester of gestation in dairy cows: comparison with the origin of embryos and featal number. Japan Agricultural Research Quarterly 37: 195-200.
Hung NN, Prakash BS (1990) Influence of gestation on blood plasma concentration of oestrone and oestrone sulphate in karan swiss cows and Murrah buffuloes. Brazilian Veterinary Journal 146: 449-456.
Janeway CA, Travers P, Walport M, Shlomchik M (2002) Immunobiologia: O sistemaimunenasaude e nadoenca. Artmed, Porto Alegre.
Levkut M, Pistl J, Revajova V, Choma J, Levkutova M, David V(2002) Comparison of immune parameters in cows with normal and prolonged involution time of uterus. Veterinarni Medicina - UZPI (Czech Republic) 47 : 277-282.
Liberg P (1977) Agarose gel electrophoretic fractionation of serum proteins in adult cattle. A study of clinically healthy cows. Acta Veterinaria Scandinavica 18:40-53.
Malek A, Sager R, Kuhn P, Nicolaides KH, Schneider H (1996) Evolution of Maternofetal transport of immunoglobulins during human pregnancy. American Journal of Reproductive immunology, 36:248-255.
Mekonnin AB, Howie AF, Riley SC, Gidey G, Tegegne DT, Desta G, Ashebir G, Gebrekidan B, Harlow CR (2017) Serum, milk, saliva and urine progesterone and estradiol profiles in crossbred (Zebu x Holstein Friesian) dairy cattle. Animal Husbandry, Dairy and Veterinary Science 1: 1-10. DOI: 10.15761/AHDVS.1000118
Min KR, Munarriz N, Kim N, Goldstein I, Traish A (2002) Effects of ovariectomy and vaginal lubrication in the animal model. American Journal of obstetrics and Gynecology187:1370-1376.
Opara MN, Okoli1 CI, Udo H, Adeyemo O (2006) Ovarian morphology and estradiol-17ß concentrations in serum and follicular fluid of slaughtered zebu cattle in Ibadan, Nigeria. Veterinarski Arhiv 76: 403-411.
Patel OV (1999) Plasma oestrone and oestradiol concentrations throughout gestation in cattle: relationship to stage of gestation and fetal number. Research in Veterinary Science 66:129-133.
Peters AR, Ball PJH (1995) parturition and lactation. In: Reproduction in Cattle, 2ndedn, Blackwell Science Ltd, UK, Pp. 127-144.
Gaiani R, Chiesa F, Mattioli M, Nannetti G, Galeati G (1984) Androstenedione and testosterone concentrations in plasma and milk of cow throughout pregnancy. Journal of Reproduction and Fertility 70: 55-59.
Snedecor GW, Cochran WG (1994) Statistical Methods. 8th Ed.,
Solomon S (1994) The primate placenta as an endocrine organ: steroids. In: Knobil E, Neill JD (Eds.), The physiology of reproduction, Raven Press, New York, USA, Pp. 863-873.
Tietz NW (1982) Fundamental of clinical chemistry. In: Norbert WT (Ed.), Sounders Company, Philadelphia.
Wyk VN., Van Ande RJ, Lauw AI (1994) Concentrations of plasma total and unbound progesterone and testosterone during pregnancy in Cape Porcupines. Comparative Biochemistry and Physiology 108A:265-271.