Volume 7, Issue 2, April Issue - 2019, Pages:222-232
|Authors: Samyah Jastaniah, Sawsan Dawalibi, Reda Amasha, Magda Aly|
|Abstract: For decades, antibiotics are wonder drugs which treat different microbes and human pathogens such as pneumonia, tuberculosis and gonorrhea which are harder and difficult to treat. Due to miss use of antibiotics in agriculture and animal husbandry, antibiotics are becoming less effective and microbes became more resistant. This resistant increased every second, thus this study aimed to produce active antibiotic from soil actinomycetes which might play a highly significant role in medicine. About 15 bacterial isolates were obtained on starch nitrate agar medium from different soil samples. They were screened for antibacterial production against 5 different human pathogens, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis and Methicillin-resistant Staphylococcus aureus (MRSA). The most active isolate was SD5 which showed the highest inhibition against E. coli, E. faecalis and MRSA was morphologically examined and characterized. Using molecular identification technique, it was reported that isolate SD5 belonging to genus Streptomyces and was similar to Streptomyces geysiriensis with 97% similarity and to Streptomyces sp. JSM147777 with 95% similarity. Maximum production of the antimicrobial agent was determined by measuring the diameter of inhibition zone using starch nitrate broth, prepared at pH 6.5 and incubation temperature at 30°C for 5 days. The antimicrobial agent was extracted by using three different organic solvents (ethyl acetate, n- Butanol and Petroleum ether). The best solvent was ethyl acetate which gave maximum inhibition against E. faecalis, E. coli and Methicillin-resistant S. aureus. In conclusion, actinomycetes especially genus Streptomyces obtained from soil still contained new isolates with excellent antimicrobial activities. Ethyl acetate is a good solvent for antimicrobial agent extraction (MIC ranged from 75-100 µg/ml) and no toxicity was recorded for all tested organic extracts at the different concentrations using Artemia salina as test organism. Purification and characterization of the antimicrobial agent must be carried out to obtain new material active against Methicillin-resistant S. aureus.|
Agwa A, Aly MM, Bonaly R (2000) Isolation and characterization of two Streptomyces species produced non polyenic antifungal agents. Journal Union Arab of Biology 7 B: 62- 84.
Aliero AA, Adam AS, Ntulume I, Bagudo AI, Kudu A, Ondieki MC, Jega SA, Odda J, Okech MA (2018) Molecular characterization and optimization of bioactive compounds production of three actinomycetes spp isolated from waste dump soil from Western Uganda. Current Trends in Biotechnology and Pharmacy, 12: 230-244.
Aly MM, El-Sabbagh SM (2004) Nile-water sediments as a source of actinomycetes exhibiting biomedical activity. Proceedings 3th International Conference Biological Science, Tanta University, Egypt, 27-28 April, 2004.
Aly MM, Gumgumjee NM (2011) Antimicrobial efficacy of Rheum palmatum, Curcuma longa and Alpinia officinarum extracts against some pathogenic microorganisms. African Journal of Biotechnology 10: 12058-12063.
Aly MM, Sanaa Totk, Saleh M. Al-Garni, Reda Allam (2013) Production and characterization of uricase from Streptomyces exfoliates UR10 isolated from farm wastes. Turkish Journal Biology 37: 520-529.
Aly MM, Tork S, Al Garni S, Nawar L (2012) Production of lipase from genetically improved Streptomyces exfoliates LP10 isolated from oil contaminated soil. African Journal of Microbiology Research 6: 1125-1137.
Aly MM, Tork S, Al-Garni S M, Kabli S A (2015) Production and characterization of phytase enzyme from Streptomyces luteogrises R10isolated from decaying wood sample. International Journal Agriculture and Biology 17:–515–522.
Amer S, Aly MM, Sabbagh S (2006). Biocontrol of dermatophytes using some plant extracts and actinomycetes filtrates. Egyptian Journal Biotechnology 14: 291--315.
American Thoracic Society and Infectious Diseases Society of America (2005) Guidelines for the management of adults with hospital-acquired, ventilator-associated, and healthcare-associated pneumonia. American Journal of Respiratory and Critical Care Medicine 172 :1169-227.
Arifuzzaman M, Khatun MR, Rahman H (2010) Isolation and screening of actinomycetes from sundarbans soil for antibacterial activity. African Journal of Biotechnology 9: 4615-4619.
Berdy J (2005) Bioactive microbial metabolites. Journal of Antibiotic (Tokyo) 58: 1-26.
Bonnavero V, Chevaleier J, Cremieux A (1998) A Rapid Method Outomatized Method for Assyment of the Fungal Activity of Natural and Synthetic Agents. In 6th Congress mediterania of Chemotherapy, Taormina, Italy, Pp. 22 27.
Dhanasekaran D, Raja-Kumar G, Sivamani P, Sivamani N, Paneerselvam A, Thajuddin N (2005) Screening of salt pans actinomycetes for antibacterial agents. International Journal Microbiology 1:6-12.
Ebadi S, Sohrabi H, Peymani A, Sarookhani M R (2018) Identification of antibiotic-producing streptomyces species in Iran’s soil by phenotypic and genotypic methods. Biotechnology and Health Sciences 5:e59854.
Frieden T (2013) Antibiotics resistance threats in the United State. Centers for Disease Control and Prevention, U. S Department of health and human services.
Gamakaranage SSK, Rodrigo C, Weerasinghe S, Gnanathasan A, Puvanaraj V, Fernando H (2011) Complications and management of acute copper sulphate poisoning; a case discussion. Journal of Occupational Medicine and Toxicology 6:34.
Ghanem NF, Aly MM (2003) Antitumor activity of Streptomyces (AFS99) against the induced-liver tumorigeneses in mice. Journal Medical Research Institute 24: 88-106.
Helmi NR, Zaman RM, Aly MM (2013) Prevalence of gram-positive bacteria in Jeddah, Kingdom of Saudi Arabia: Study of antimicrobial resistance patterns and molecular typing. International Journal Pharm Bio Science 4: 1231 – 1245.
Hindler JA, Inderlied CB (1985) Effect of the source of Mueller-Hinton agar and resistance frequency on the detection of methicillin-resistant Staphylococcus aureus. Journal Clinical Microbiology 21: 205–210.
Hyun DY, Mason EO, Forbes A, Kaplan SL (2009) Trimethoprim-sulfamethoxazole or clindamycin for treatment of community acquired methicillin-resistant Staphylococcus aureus skin and soft tissue infections. Pediatric Infectious Disease Journal 28:57-9. 20.
Isaacson PJ, Webster JM (2002) Antimicrobial activity of Xenorhabdus sp. RIO (Enterobacteriaceae), symbiont of the entomopathogenic nematode, Steinernema riobrave (Rhabditida: Steinernematidae). Journal of Invertebrate Pathology 79:146–153.
Janardhan A, Kumar A, Viswanath B, Saigopal DVR, Narasimha G (2014) Production of bioactive compounds by actinomycetes and their antioxidant properties. Biotechnology Research International, Article ID 217030, 8 pages.
Jeffrey LS (2008) Isolation, characterization and identification of actinomycetes from agriculture soils at Semongok, Sarawak. African Journal Biotechnology 7: 3697-3702.
Jiao W, Zhang F, Zhao X, Hu J, Suh JW (2013) A novel alkaloid from marine-derived actinomycete Streptomyces xinghaiensis with broad-spectrum antibacterial and cytotoxic activities. PLoS ONE 8(10): e75994. doi:10.1371/journal.pone.0075994.
Kaur DC, Chate SS (2015) Study of Antibiotic Resistance Pattern in Methicillin Resistant Staphylococcus Aureus with Special Reference to Newer Antibiotic. Journal of global infectious diseases 7:78-84.
Kaur DC, Narayan PA (2014) Mupirocin resistance in nasal carriage of Staphylococcus aureus among healthcare workers of a tertiary care rural hospital. Indian Journal of Critical Care Medicine 18:716-21.
Kekuda TRP, Shobha KS, Onkarappa R (2010) Fascinating diversity and potent biological activities of Actinomycetes metabolites. Journal of Pharmacy Research 3: 250–256.
Koehn FE, Carter GT (2005) The evolving role of natural products in drug discovery. Nature Reviews Drug Discovery 4: 206–220.
Kumar N, Singh RK, Mishra SK (2010) Isolation and screening of soil actinomycetes as sources of antibiotics active against bacteria. International Journal of Microbiology Research 2: 12 -16.
Laverde Gomez JA, Hendrickx AP, Willems RJ, Top J, Sava I, Huebner J, Witte W, Werner G (2011) Intra- and interspecies genomic transfer of the Enterococcus faecalis pathogenicity island. PLoS One 6 : e16720.
Lee DS, Lee SJ, Choe HS (2018) Community-acquired urinary tract infection by Escherichia coli in the era of antibiotic. BioMed Research International 7656752. DOI: https://doi.org/10.1155/2018/7656752.
Lucas CP, Oliveira R, Neto J, Schmidt R, Salvi C, da Silva F (2019) Evaluation of cytotoxic and mutagenic activities of Tabebuia aurea (Silva Manso). IOSR Journal Of Pharmacy 9: 62-69.
Luna CM, Noriega ER, Bavestrello L, Gotuzzo E ( 2010) Treatment of methicillin-resistant Staphylococcus aureus in Latin America. Brazilian Journal of Infectious Diseases 14 (Suppl. 2): S119-S127.
Meyer BN, Ferrigni NR, Putnan JE, Jacobs LB (1982) Brine shrimp: a convenient general bioassay for active plant constituents. Planta Medica 45: 31-34.
Moran GJ, Krishnadasan A, Gorwitz RJ, Fosheim GE, Albrecht V, Limbago B, Talan DA (2006) Methicillin-resistant S. aureus infections among patients in the emergency department. New England Journal of Medicine 355:666-74.
Naine J, Srinivasan MV, Devi SC (2011) Novel anticancer compounds from marine Actinomycetes : a review. Journal of Pharmacy Research 4: 1285–1287
NCCLS (2002) Reference method for broth dilution antifungal susceptibility testing of yeasts; Approved Standard—Second Edition. NCCLS document M27-A2 (ISBN 1-56238-469-4), NCCLS, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA.
Omura S, Takahashi Y, Iwai Y, Tanaka H (1982) Kitasatosporia, a new genus of the order Actinomycetales. Journal of Antibiotic 35:1013–1019.
Oskay M, Usame A, Azeri C (2004) Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. African Journal Biotechnology 3: 441- 446.
Otsuki T, Kumar S, Kingma DW, Yano I, Stetler-Stevenson M, Jaffe ES, Raffeld M (1996) Detection of HHV-8/KSHV DNA sequences in AIDS-associated extranodal lymphoid malignancies. Leukemia 10: 1358-1362
Pandey B, Ghimire P, Agrawal VP (2004) Studies on the antimicrobial activity of actinomycetes isolated from Khumbu region of Nepal. Ph. D. Thesis submitted to the Tribhuvan University, Kathmandu, Nepal.
Peacock SJ, Paterson GK (2015) Mechanisms of Methicillin Resistance in Staphylococcus aureus. Annual Review Biochemistry 84:577-601.
Rabah S, Azab E, Aly MM (2007) Studies on bacterioplankton and inhibitory strains of actinomycetes in lake Bardawil, Egypt. World Journal Microbiology Biotechnology 23:111-116.
Rabah S, Azab E, Aly MM (2006) Efficiency of waste stabilization pond in removing some pathogenic bacteria and the possible role of aquatic actinomycetes. New Egyptian Journal Microbiology 15: 186-203.
Sader HS, Moet GJ, Jones RN (2009) Antimicrobial resistance among Gram-positive bacteria isolated in Latin American hospitals. Journal Chemotherapy 21: 611-20.
Salami F (2004) Isolation and determination of Streptomyces that produce antibiotic from soil. Pajohesh-ve-Sazandegi 17:41-47.
Sangian H, Faramarzi H, Yazdinezhad A, Mousavi SJ, Zamani Z, Noubarani M (2013) Antiplasmodial activity of ethanolic extracts
Shirling EB, Gottlieb D (1966) Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study. International Journal Systematic Bacteriology18:169-89.
Shittu AO, Udo EE, Lin J (2009). Phenotypic and molecular characterization of Staphylococcus aureus isolates expressing low- and high-level mupirocin resistance in Nigeria and South Africa. BMC Infectious Diseases 9:10-19.
Tork SE, Aly MM, Elsemin O (2018) A new l-glutaminase from Streptomyces pratensis NRC 10: Gene identification, enzyme purification, and characterization. International Journal of Biological Macromolecules 113:550-557.
Torsvik V, Ovreas L (2002) Microbial diversity and function in soil: from genes to ecosystems. Current Opinion in Microbiology 5: 240 – 245.
Wang Y, Fang X, Cheng Y, Zhang X (2011) Manipulation of pH shift to enhance the growth and antibiotic activity of Xenorhabdus nematophila, Journal of Biomedicine and Biotechnology Article ID 672369, 9 pages.
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173 :697-703.
Westley JW, Evans RH, Sello LH, Troupe N, Liu CM, Blount JF (1979) Isolation and characterization of antibiotic X-14547A, a novel monocarboxylic acid ionophore produced by Streptomyces antibiotics NRRL 8167. Journal Antibiotic 32: 100–107.
Woodward S (2015) E. coli: A brief overview. British journal of nursing 24:158-160.
Yang X, Yang W, Jian H (2001) Effect of fermentation conditions on antibiotic production of Xenorhabdus nematophilus. Chinese Journal of Microbiology 28: 12–16.
Zaman R M, Aly M M and Helmi N R (2015) Antimicrobial susceptibility pattern of Gram-negative bacilli isolated from a Teaching Hospital in Jeddah, Saudi Arabia. African Journal of Biotechnology 9: 2145-2158.