Volume 7, Issue 1, February Issue - 2019, Pages:95-102
|Authors: Chandravathany Devadason|
|Abstract: The salmon louse, Lepeophtheirus salmonis, is a crustacean ectoparasite of salmonids and causes significant economic losses in Atlantic salmon farms. This study investigated the cellular inflammatory response, specifically macrophage migration, to the attachment of salmon louse to the epidermis of Atlantic salmon (Salmo salar). During the inflammatory response, macrophage infiltration is common at the site of infection in the host macrophages possessing phagocytic activity. The aim of the study was to investigate the immunological response of macrophages to the parasitic excretory and secretory product (E/S). The E/S of the parasites collected from the explants and in vitro cultures of parasites and macrophages isolated from fish blood were used. A macrophage migration and a phagocytosis assay were performed to study the inflammatory response. The inhibition of the macrophage migration response (p<0.05) and a significantly lower level of phagocytosis (p<0.05) were observed. The results indicate that the anti-inflammatory response of the host is elicited during louse infection.|
Bartos JM, Sommer CV (1981) In vitro cell mediated immune response to M. tuberculosis and M. salmoniphilumin rainbow trout, Salmo gairdneri. Developmental and Comparative Immunology 5: 575-83.
Bayne CH, Meino JS, Hobbs D, Barnes DW (1994)In vitro cultivation of cells from larval Schistosoma mansoni. Journal of Parasitology 80: 29-35. DOI.10.2307/328334.
Boydon S (1962) The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. Journal of Experimental Medicine 115: 453-460.
Cawthorn R, Backman S, Groman D, O’Halloran J, Johnson G (1990) Dermocystidium-like parasite in farmed Atlantic salmon. Canadian Veterinary Journal Review 31: 591.
Chang G, Gabrielson DA (1984) Toxoplasma gondii: growth in ovine fetal kidney cell cultures. Experimental Parasitology 57 : 81-85.
Clem LW, Sizemore RC, Elsaesser CF, Miller NW (1985) Monocytes as accessary cells in fish immune responses. Developmental and Comparative Immunology 9: 803-809.
Creamer HR, Hunter N, Bullock WW, Gabler WL (1991) Concurrent lipopolysaccharide enhances chemotactic response of human polymorphonuclear leucocytes to bacterial chemotaxis. Inflammation 15: 201-211.
Cuha FQ, Ferreira SH (1986) The release of a neutrophil chemotactic factor from peritoneal macrophages by endotoxin inhibition by glucocorticoids. European Journal of Pharmacology 129: 65.
Dinarello CA (1991) The pro-inflammatory cytokines interleukin-1 and tumor necrosis factor in septic shock syndrome. Journal of Infectious Diseases 163: 1177-1184.
Ellis AE, Munro ALS, Roberts RJ (1976) Defense mechanisms in fish: A study of the phagocytic system and the fate of intraperitonially injected particulate material in the plaice (Pleuronecte splatessa L). Journal of Fish Biology 8: 67-78.
Fusco AC, Salafsky B, Delbrook K (1986) Schistosoma mansoni, Penetration of cercarial eicosanoids as correlates of penetration and transformation. Journal of Parasitology 79: 444-448.
Griffin BR (1984) Random and directed migration of trout (Salmo gairdneri) leucocytes. Developmental & Comparative Immunology 7:253-259.
Hughes HPA, Hudson L, Fleck DG (1986) In vitro culture of Toxoplasma gondii in primary and established cell lines. International Journal of Parasitology 16: 317-322.
Jensen JB, Edger SA(1976) Possible secretory function of the rhoptries of Eimeria magna during penetration of cultured cells. Journal of Parasitology 62: 988-992.
Johnson SC, Albright LJ (1992a) Comparative susceptibility and histopathology of the response of naïve Atlantic, Chinook and Coho salmon to experimental infection with Lepeophtherius salmonis (Cpepoda: Caligidae). Diseases of Aquatic Organisms14: 179-193.
Johnson SC, Albright LJ (1992b) Developmental stages of Lepeophtherious salmonis (Kroyer, 1837, Copepod: Caligidae). Canadian Journal of Zoology 69: 929-950.
Jones SRM, Woo PTK (1986) The immune response of rainbow trout, Salmo gairdneri Richardson to the haemoflagellate Cryptobia salmostitica Katz 1951. Journal of Fish Diseases 15:521-527.
Kirkpatrick CJ, Svilenov D (1987) Studies on the adhesion of protoscoleces from Echinococcus multicularis and E. granulosus to artificial substrates and human endothelial cells in vitro. Parasitology Research74: 61-68.
Knight J, Rowley AF (1995)Immunoregulatory activities of eicosanoids in the rainbow trout (Oncorhynchus mykiss). Immunology 85:389 – 393.
Ko RC (1989)Current Concepts in Parasitology. Hong Kong University Press, Hong Kong.
Komano H, Kasama E, Nakanishi Y, Matsuyama K, Ando K, Nagasawa Y, Natori S (1988) Synthesis of Sarcophaga lectin and sarcotoxins in NIH-SAPE-4, an embryonic cell line of Sarcophaga peregrine. In: Kuroda Y, Kurstak E,Maramorosch K (Eds). Invertebrate and Fish Tissue Culture, Japan Societies Press, Tokyo
Kusuda R, Salati F, Hamaguchi M, Kawai K (1991) The effect of triploidy on phagocytosis, leucocyte migration, antibody and complement levels of ayu, Pleoglossusaltivelis. Fish and Shellfish Immunology 1: 243-249.
Lee H, Hou RF (1992) Establishment of a cell line derived from embryos of the diamond back moth, Plutella xylostella (L). Journal of Invertebrate Pathology 59: 174-177.
Luedeman RA, Lightner DV (1992) Development of an in vitro primary cell culture system from the penaeid shrimp, Penaeus stylirostris and Penaeus vannami. Aquaculture 101: 205-211.
Lumb R, Smith K, O’Donoghue LLA (1988) Ultrastructure of the attachment of Cryptosporium sporozoites to tissue culture cells. Parasitic Research 74: 531-536.
MacArthur JI, Fletcher TC (1985) Phagocytosis in fish. In: Manning MJ, Tatner MF (Eds.), Fish Immunology, Academic Press, London.
Maramorosch K (1987) Biotechnology in Invertebrate Pathology and Cell Culture. Academic Press, San Diego.
McVicar AH (1975) Infection of plaice Pleuronectes platessa L. with Glugea (Nosema) stephani (Hagenmuller,1899) (Protozoa:Microsporidea ) in a fish farm and under experimental conditions. Journal of Fish Diseases 7:611-619.
Munderloh UG, Liu Y, Wang M, Chen C, Kurtti TJ (1994) Establishment, maintenance and description of cell lines from the tick, Ixodes scapularis. Journal of Parasitology 80: 533-543.
Nakai Y, Tsuchiya H, Takahashi S (1992) Cultivation of Eimeria tenella in Japanese quail embryo. Journal of Parasitology78: 1024-1026.
Paperna L, Zwerner DE (1982) Host-parasite relationship of Ergasilus labracis Kroyer (Cyclopidea, Ergasilidae) and the striped bass, Morone saxatilis (Walbaum) from the lower Chesapeake Bay. Annales De Parasitologie Humaine Et Comparee 57: 393-405
Phillips HJ (1973) Dye exclusion tests for cell viability. In: Kruse PK, Patterson MK (Eds.), Tissue Culture Methods and Application, Academic Press, New York.
Pike AW (1989) Sea lice – Major pathogens of farmed Atlantic salmon. Parasitology Today 5: 291-297.
Rae GH (1979) On the trail of the sea louse. Fish Famer 2: 22-25.
Ribeiro LP, Ahne W, Lichtenberg V (1983) Primary culture of normal pituitary cells of carp (Cyprinus carpio) for the study of gonadotropin release. In Vitro19: 41-45. DOI. 10.1007/BF0261799.
Rowley AF (1991) Lipoxin formation in fish leucocytes. Biochimica et Biophysica Acta1084: 303-306.
Samuelsson B (1983) Leukotrienes: mediators of immediate hypersensitivity reactions and inflammation. Science 229: 568.
Secombes CJ, Chung S, Jeffries AH (1988) Superoxide anion production by rainbow trout macrophages detected by the reduction of ferricytochrome. Developmental Comparative Immunology 12: 201-206.
Shariff M, Roberts R (1989) The experimental histopathology of Lernaeapolymorpha Yu, 1983 infection in naïve Aristichthysnobilis (Richardson) and a comparison with the lesion on naturally infected clinically resistant fish. Journal of Fish Diseases12: 405-414.
Smith KA, Lachman LB, Oppenheim JJ, Favata MF (1980) The functional relationship of the interleukins. Journal of Experimental Medicine151: 1551.
Speer CA, Dubey JP (1986) Vascular phase of Sarcocystis cruzi cultured in vitro. Canadian Journal of Zoology 64: 209-211.
Taylor AER, Baker JR (1978) Methods of Cultivating Parasites in vitro. Academic Press, London.
Weissenberg R (1968) Intracellular development of the microsporidian Glugeaanomala in hypertrophying migratory cells of fish Gasterosteus aculeatus: an example of the formation of xenoma tumors. Journal of Protozoology 15: 44-57.