Volume 6, Issue 1, February Issue - 2018, Pages:243-248
|Authors: Arna Das, Sarita K. Pandey, Pradipta Bhattacharya, T. Dasgupta|
|Abstract: Seed storage protein markers being less sensitive to environmental fluctuation than phenological traits, has been successfully employed in assessing divergence in many crop plants. The present study was aimed to find out correlation of seed storage protein markers in twenty eight Indian sesame cultivars with their agro-ecological zone of adoption and their seed coat colour. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) revealed altogether twenty two protein bands of which thirteen were polymorphic with varied molecular weights. Specific bands, relating to specific agro-ecologies were found. Moreover, bands of 93.40 KDa and 68.05 KDa were found associated with production of darker shades of seed coat colour. Clustering pattern based on protein similarity value offered no definite grouping, either to specific agro-ecological zones of adoption or to specific seed coat colour. It is concluded that individual protein banding pattern can be linked to agro-ecological adoption zone and seed coat colour which is helpful in divergence and phylogenetic study in sesame.|
|Full Text: 1 Introduction Sesame (Sesamum indicum L.) as an oilseed is potent to meet the domestic demand of edible oil in India. Though India is one of the major sesame producers in the world (www.faostat.fao.org - 2016), but this crop has been highly neglected and identified as an orphan crop. This resulted into enormous loss of germplasm and drastic reduction in variation. An insight into characterization and preservation of naturally existing variation in sesame, therefore, has become a necessity for further improvement of the crop. Phenological traits, due to pleiotropic effect and polygenic control, exhibit overlapping variation within and between species populations offering taxonomic complexity (Huber-Morath & Phlomis, 1982; Wang et al., 2010; Pabby & Rockman, 2013). Soluble seeds storage protein markers assessed through Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) had been successfully employed to characterize cultivated varieties in several crop plant species, of which Mung (Ghaffor et al., 2002), Pea (Jha & Ohri, 2002), Einkorn wheat (Alvarez et al., 2006), Brassica (Khurshid & Rabbani, 2012) are few to name. Reliability of soluble seed storage protein markers lies in the fact that these are less influenced by environmental fluctuation as compared to phenological markers, therefore stable, uniform and reproducible. Moreover SDS-PAGE takes less time, is simpler to perform and is more economic than nucleic acid markers. Only a few references are available on soluble seeds storage protein polymorphism study involving Indian sesame germplasm. Akhila & Beevy (2011) reported the presence of fourteen bands in a study of seven sesame genotypes including wild and cultivated species. The protein polymorphism was able to group the genotypes belonging to two different species into separate clusters. Das et al. (2013) reported the presence of twenty two bands in a study with twenty six advanced sesame mutant lines and respective controls. But the study did not offer much specificity in grouping the mutants according to their parental origin. Dar et al., (2014) reported as much as twenty one bands in a study with fifty two Indian sesame germplasms. Similarly, Singharaj & Onsaard (2015) carried out SDS-PAGE in sesame genotypes with varied seed coat colour, for its food value. According to recent study, seed coat colour in sesame is more helpful in phylogenetic study than geographic origin of genotypes (Zhang et al., 2013). In the present research work an attempt was made to search for correlation between soluble seed storage protein polymorphism of a number of Indian sesame cultivars with the cultivars’ agro-ecological zone of adoption and also with cultivars’ seed coat colour, which would definitely incite more knowledge on divergence and phylogeny in sesame. Correlation of protein markers with seed coat colours would further help in assessing genotypes for those biochemical traits which are linked to particular seed coat colours (Zhang et al., 2013). Such correlation, if exists, can be employed to identify diverse and superior sesame genotypes for future crop improvement programme. 2 Materials and methods |
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