Volume 6, Issue 1, February Issue - 2018, Pages:211-219
|Authors: Zahran F, Gabr SA*, Abd El-Moneim AE, Sharoud MN,Hassanin.WF, Mesalam NM|
|Abstract: Present study has been carried out evaluate prophylactic effect of Camel milk on physiological and biochemical changes in CCl4-intoxicated rats. A total of 36 male albino rats were randomly divided into 6 groups viz. control group (G1), silymarin (SM) group (G2), camel milk (CM) group (G3), CCl4-intoxicated group (G4), silymarin prophylactic group (G5) and camel milk prophylactic group (G6). Each group has six rats. After completion of the study (8 wks), blood samples were collected and hematological parameters, liver contents of glutathione (GSH), malondialdhyde (MDA) , superoxide dismutaseactivity (SOD), serum urea, uric acid, creatinine, testosterone, estradiol were measured. The results of this study revealed that CCl4 toxicity significantly reduced Hb level, RBCs count and PCV, whereas it significantly increased the WBCs count with respect to normal control. Intoxication of CCl4 in G4 rats led to microcytic hypochromic anaemia, increased erythrocyte fragility and, leukocytosis was accompanied neutrophils increases and a decrease in lymphocyte counts. Also, CCl4 produced significant increase in serum urea, uric acid, creatinine and estradiol level and decrease in circulating testosterone level compared with normal rats. Pre-treatment with CM and SM brought significant restoration in hematological, renal function parameters and sexual hormones disturbance. Moreover, lipid peroxidation and oxidative stress were obviously noted in CCl4 intoxicated (G4) organism where a significant increase in liver MDA and decrease in its content of GSH and SOD activity. Contrarily, pre-treatment with CM and SM did not only decrease liver content of MDA but also increased hepatic GSH and SOD activity, suggesting that CM and SM attenuated CCl4-induced oxidative stress. In conclusion, CM and SM had a considerable prophylactic effectiveness against hematotoxicity, oxidative stress, lipid peroxidation, renal impairment and sexual hormones disturbances developed by intraperitoneal injection of rats withCCl4.|
Abdel Kader SM (2017) Effect of Salvia aegyptiaca Aqueous Extract on Some Neurohormonal Disorders Induced by Carbon Tetrachloride in Adult Male Albino Rats. Arab Journal of Nuclear Sciences and Applications 50: 205-216.
Abdel- Rahman M, El-Nahary H (2004) Therapeutic and protective role of panaxgensing extract on some neurotransmitters in rats treated with CCl4. Bullettin Pure and Applied. Science 23: 71-86.
Abdel-Moneim AM, Al-Kahtani MA, El-Kersh MA, Al-Omair MA (2015) Free Radical-Scavenging, Anti-Inflammatory / Anti-Fibrotic and Hepatoprotective Actions of Taurine and Silymarin against CCl4 Induced Rat Liver Damage. PLoS ONE 10(: e0144509). DOI: https://doi.org/10.1371/journal.pone.0144509.
Adewole SO, Salako AA, Doherty OW, Naicker T ( 2007) Effect of melatonin on carbon tetrachloride-induced kidney injury in Wistar rats . BMC Public Health 10: 153-64.
Al-Shamsi M, Amin A, Adeghate E (2006) Effect vof vitamin c on liver and kidney functions in normal and diabetic rats. Annals of the New York academic of Sciences 10 : 371 – 390.
Althnaian T (2012) Protective Effect of Camel Milk Against Carbon Tetrachloride Hepatotoxicity in Rats. Global Veterinaria 9 : 564-570.
Baker HJ, Lindsey JR, Weisbroth SH (1979) Housing to control research variables. In: the laboratory rats. Biological and Diseases, Academic Press, New York.
Banfi G, Del Fabbro M (2006) Relation between serum creatinine and body mass index in elite athletes of different sport disciplines. British Journal of Sports Medicine 40:675–8.
Barbagallo M, Dominguez LJ, Tagliamonte MR, Resnick LM, Paolisso G (1999) Effects of Vitamin E and Glutathione on Glucose Metabolism Role of Magnesium. Hypertension 34:1002-6.
Barham D, Trinder P (1972) An improved colour reagent for the determination of blood glucose by the oxidase system. Analyst 97: 142–145.
Bektur NE, Sahin E, Baycu C, Unver G (2016) Protective effects of silymarin against acetaminohpen-induced hepatotoxicity and nephrotoxicity in mice. Toxicology and Industrial Health 32:589-600.
Beutler E, Duron O, Kellin BM (1963) Improved method for the determination of blood glutathione.Journal of Laboratory and Clinical Medicine 61: 882–888.
Cecen E, Dost T, Culhaci N, Karul A, Ergur B, Birincioglu M (2010) Protective effects of silymarin against doxorubicin-induced toxicity. Asian Pacific Journal of Cancer Prevention 12 : 2697–704.
Chen IS, Chen YC, Chou CH, Chuang RF, Sheen LY, Chirinu CH (2012) Hepatoprotection of silymarin against thioacetamide-induced chronic liver fibrosis. Journal of the Science of Food and Agriculture 92 : 1441-7.
Churchill DN, Finn A, Gault M (1983) Association between hydrocharbon exposure and glomerulonephritis. Anaraisal of the evidence. Nephron Journal, 33: 169-172.
Curney ME, Cutting FB, Zhai P (1996) Benefit of vitamin E, riluzole and gabapentin in a transgenic model of familial amyotrophic lateral sclerosis. Annual Neurology 39: 147–157.
Dacie JV, Lewis MS (1993) In practical hematology 6th ed., ch 5, Churchill Livings ton, London and N.Y., Pp. 37.
Duncan DB (1955) Multiple range and multiple F. test. Biometrics 11,1.
El Miniawy HMF, Ahmed KA, Mansour S, Khattab MMS (2017) In vivo antitumour potential of camel’s milk against hepatocellular carcinoma in rats and its improvement of cisplatin renal side effects. Pharmaceutical Biology 55:1513-1520.
El Miniawy HMF, Ahmed KA, Tony MA, Mansour S, Khattab MMS (2014) Camel milk inhibits murine hepatic carcinogenesis, initiated by diethylnitrosamine and promoted by phenobarbitone. International Journal of Veterinary Science and Medicine 2: 136–141.
El-Agamy SI, Ruppanner R, Ismail A, Champagne CP, Assaf RJ (1992) Antibacterial and Antiviral activity of camel milk protective proteins. Journal of Dairy Research 59: 169-175.
Farah Z, Rettenmaier R, Atkins D (1992) "Vitamin content of camel milk". International journal for vitamin and nutrition research 62:30-33.
Fawcett JK, Scott JE (1960) A Rapid And Precise Method For The Determination Of Urea. Journal of Clinical Pathology 13: 156–159.
Hai ZH, Bing W, Yong KL, Yong YB, Yan GU (2011) Hepatoprotective and Antioxidant Effects of Licorice Extract against CCL4-Induced Oxidative Damage in Rats. International Journal of Molecular Sciences 12: 6529-6543.
Hamed MA, Ali SA, El-Rigal NS (2012) Therapeutic Potential of Ginger against Renal Injury Induced by Carbon Tetrachloride in Rats. The Scientific World Journal : 1-12. doi: 10.1100/2012/840421.
Ibrahim MA, Wani FA, Rahiman S (2017) Hepatoprotective effect of olive oil and camel milk on acetaminophen-induced liver toxicity in mice. International Journal of Medical Science and Public Health 6: 186-194.
Jain NC (1986) Schalm’s Veterinary Haematology 4th ed. Lea and Febiger, Philadelphia.
Khan MR, Rizvi W, Khan GN, Khan RA, Shaheen S (2009) Carbon tetrachloride-induced nephrotoxicity in rats: protective role of Digeramuricata. Journal of Ethnopharmacology 122 : 91–99.
Khan MR, Afifa M, Shabbir M, Saeed N, Jasia B (2012) Antioxidant and hepatoprotective effects of oxalis corniculata against carbon tetrachloride (CCl4.) induced injuries in rats. African Journal of Pharmacy and Pharmacology 6 : 2255-2267.
Kim SH, Yang YP, Sung SH, Kim CJ, Kim JW, Kim YC (2003) Hepatoprtective of dibenzylbuyrolactoneligans of Torreyanuciferaagainst CC14- induced toxicity in primary cultured rat hepatocytes. Biological and Pharmaceutical Bulletin 26 :1202-1205.
Knockaert L, Berson A, Ribault C, Prost PE, Fautrel A, Pajaud J, Lepage S, Lucas-Clerc C, Bégué JM, Fromenty B, Robin MA (2012) Carbon tetrachloride-mediated lipid peroxidation induces early mitochondrial alterations in mouse liver. Laboratory Investigation 92: 396–410.
Knoess KH ( 1979) Milk Production of the Dromedary. In the Proceeding of the 1st (IFS ) International Symposium on Camels, (ISC’79), Sudan, Pp:201-202.
Korhonen H, Pihlanto A (2001) Bioactive peptides opportunities for designing future foods. Current Pharmaceutical Design 9: 1297–1308.
Kraus A, Roth HP, Kirchgessner M (1997) Supplementation with vitamin C, vitamin E or beta-carotene influences osmotic fragility and oxidative damage of erythrocytes of zinc-deficient rats. Journal of Nutrition 127 : 1290-1296.
Kumarappan CT, Vhand BS, Mandal SC, Sengottuvel T (2010) Hepatoprotective effect of the poly phenolic extract from ichnocarpus fruits scens leaves. Deccan Journal of Pharmacology 1 : 1-16.
Larsen K (1972) Creatinine assay by a reaction-kinetic principle. Clinica Chimica Acta 41:209-17.
Latif R, Lodhi GM, Aslam M (2008) Effects of amlodipine on serum testosterone, testicular weight and gonado-somatic index in adult rats,” Journal of Ayub Medical College, Abbottabad 20: 8–10.
Makni M, Yassine C, Hamadi F, El Mouldi G, Mohamed B, Chama M, Choumous K, Najiba Z (2012) Erythrocyte oxidative damage in rat treated with CCl4 protective role of vanillin. Saga Journal 28: 908-916.
Mandal A, Karmakar R, Bandyopadhyay S, Chatterjee M (1998) Antihepatotoxic potential of Trianthema portulacastrum in carbon tetrachloride-induced chronic hepatocellular injury biochemical characteristics. Archives of Pharmacal Research 21: 223-230.
Manibusan MK, Odin M, Eastmond DA (2007) Postulated carbon tetrachloride mode of action: A review. Journal of Environmental Science and Health 25: 185–209.
Maxine M, Benjamin BS (1985) Outline of veterinary clinical pathology (3rdEdn) Rakha printers PVT. LTD. New Delhi.
Mayuren C, Reddy VV, Priya SV, Devi VA (2010) Protective effect of Livactine against CCl4 and paracetamol induced hepatotoxicity in adult Wistar rats. North American Journal of Medical Sciences 2:491- 495.
McDowell L (2000) Vitamins in Animal and Human Nutrition .2nd ed. Iowa State Univ. Press. Iowa, USA., Pp: 155-225.
Nishikimi M, Roa NA, Yogi K (1972) The occurrence of superoxide 728 anion in the reaction of reduced phenazinemetho sulfate and molecular oxygen. Biochemical and Biophysical Research Communications, 46: 849–854.
Omar TY, Hmmam FH (2014) Effect of Camel Milk on Hepatic Antioxidant Enzymes in Rabbits Intoxicated with Carbon Tetrachloride. International Journal of Biological & Pharmaceutical Research 5: 461-468.
Ozturk F, Ucar M, Ozturk IC, Vardi N, Batcioglu K(2003) Carbon tetrachloride-induced nephrotoxicity and protective effect of betaine in Sprage-Dawley rats. Urology 62:353-356.
Parveen R, Baboota S, Ali J, Ahuja A, Vasudev SS, Ahmad S (2011) Effects of silymarinnano emulsion against carbon tetrachloride-induced hepatic damage. Archives of Pharmacal Research 34:767–774.
Perez AJ, Courel M, Sobrado J, Gonzalez L (1987) Acute renal failure after topical application of carbon tetrachloride. Lancet 1: 515-516.
Rao MB, Gupta RC, Dastur NN (1970) Camels' milk and milk products. Indian Journal of Dairy Science 23: 71-78.
Sahreen S, Khan MR, Khan RA (2013) Ameliorating Effect of Various Fractions of Rumex hastatus Roots against Hepato- and Testicular Toxicity Caused by CCl4. Oxidative Medicine and Cellular Longevity . 2013:1-11. http://dx.doi.org/10.1155/2013/325406.
Satoh K (1978) Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta 90:37-43.
Shaker ME, Zalata KR, Mehal WZ, Shiha GE, Ibrahim TM (2011) Comparison of imatinib, nilotinib and silymarin in the treatment of carbon tetrachloride-induced hepatic oxidative stress, injury and fibrosis. Toxicology and Applied Pharmacology 252:165–175.
Singh N, Kamath V, Narasimhamurthy K, Rajini PS (2008) Protective effect of potato peel extract against carbon tetrachlorideinduced liver injury in rats. Environmental Toxicology and Pharmacology 26 : 241-246. doi: 10.1016/j.etap.2008.05.006.
Snedecor GW, Cochran WG (1982) statistical methods. 7th ed., the lawa state university Press, Ames, Lawa, USA, Pp.215.
Soliman MM, Attia HF, Nassan MA (2016) Protective effect of camel milk on genetic and histopathological changes retated to fertility and vision in diabetic wistar rats. lucr?ri?tiin?ifice vol. 59 medicin?veterinar?partea 1 : 33-54.
Swenson MJ (1993) Physiological properties and cellular and chemical constituent of blood. In: Dukes’ Physiology of domestic animals. Comstock Publishing Associates, Ithaca and London Pp. 29-32.
Tung HT, Cook FW, Wyatt RD, Hamilton PB (1975) The anemia caused by aflatoxin. Poultry science 54: 1962-1969.
US EPA IRIS (2010) Toxicological review of carbon tetrachloride (External Review Draft). U.S. Environmental Protection Agency, Washington, DC, EPA/635/R-08/005A. Available at www.epa.gov/iris/toxreviews/0020tr.pdf accessed on 25th September 2012.
Vargas-Mendoza N, Madrigal-Santillán E, Morales-González A, Esquivel-Soto J, Esquivel-Chirino C, García-Luna Y, González-Rubio M (2014) Hepatoprotective effect of silymarin. World Journal of Hepatology 6 :144–149.
Veldink JH, Kalmijn S, Groeneveld GJ, Wunderink W, Koster A, de Vries JHM, Van der Luyt J, Wokke JHJ, Van den Berg LH (2007) Intake of polyunsaturated fatty acids and vitamin E reduces the risk of developing amyotrophic lateral sclerosis. Journal of Neurology, Neurosurgery and Psychiatry 78: 367 – 371.
Venkatanarayana G, Sudhakara G, Sivajyothi P, Indira P (2012) Protective effects of curcumin and vitamin E on carbon tetrachloride-induced nephrotoxicity in rats. EXCU Journal 11: 641-650.
Videla LA, Valenzuela A (1982) Alcohol ingestion, liver glutathione and lipoperoxidation: metabolic interrelations and pathological implications. Life Sciences - Journal 31: 2395-2407.
Yacout GA, Elguindy NM, EI Azab EF (2012) Hepatoprotective effect of basil (Ocimum basilicum L.) on CCI.-induced liver
Yagil R, Saran A, Etzion Z (1984) Camel’s milk: for drinking only? Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology 78: 263-266.
Yang BY, Zhang XY, Guan SW, Hua ZC (2015) Protective effect of procyanidin B2 against CCl4-induced acute liver injury in mice. Molecules 20: 12250–12265.
Young DS, Thomas DW, Friedman RB, Pestaner LC (1972) Effects of drugs on clinical laboratory tests. Clinical Chemistry 18:1041- 1303.
Yousef MI, Awad TI, Mohamed EH (2006) Deltamethrin – induced oxidative Damage and biochemical alterations in rat and its attenuation by Vitamin E. Toxicology 29: 240 – 247.
Youssef WI, Mullen KD (2002) The liver in other (nondiabetic) endocrine disorders. Clinical Liver Disease 6: 879-89.
Zietz B, Lock G, Plach B, Drobnik W, Grossmann J, Schölmerich J, Straub RH (2003) Dysfunction of the hypothalamic- pituitary-glandular axes and relation to Child-Pughclassification in male patients with alcoholic and virus-relatedcirrhosis. European Journal of Gastroenterology & Hepatology. 15: 495-01.