Volume 6, Issue 1, February Issue - 2018, Pages:176-187
|Authors: Srinivasa Babu T, Rathnamma. D, Shrikrishna Isloor, Chandranaik. BM, Veeregowda BM, Manjunath Reddy GB|
|Abstract: Outbreaks of ORF virus (ORFV) infection in small ruminants, which occurred in eight districts of Karnataka, India were investigated during this study. Seventy scab samples were collected from affected sheep and goats from 27 outbreaks and subjected for virus isolation and polymerase chain reaction. The ORFV was successfully isolated in the chicken embryos (CE) by CAM route of inoculation. Polymerase chain reaction (PCR) was standardized targeting B2L gene for detection of ORFV. The screening of 70 samples for B2L gene amplification revealed 60 per cent positivity of Orf infection. The phylogenetic analysis based on nucleotide and deduced amino acid sequences of full length and partial B2L gene revealed that, the ORFV isolates had highest genetic homology among them and with the other Indian ORFV isolates. The maximum genetic heterogeneity was observed with ORFV isolates from other countries. Further, full length B2L gene sequence based analysis provided significant information about the genetic relationship among the various ORFV isolates. Sequence analysis revealed high nucleotide (98.7 -100 per cent) and amino acids (98.4-99.7 per cent) identity with Indian isolates maximum with Assam/09 and Assam/10 ORFV isolates.|
Azwai SM, Carter SD, Woldehiwet Z (1995) Immune responses of the camel (Camelus dromedarius) to contagious ecthyma (Orf) virus infection.Veterinary Microbiology 47: 119-131
Bora DP, Venkatesan G, Bhanuprakash V, Balamurugan V, Prabhu M, Siva sankar MS, Yogisharadhya R (2011) Taq Man real-time PCR assay based on DNA polymerase gene for rapid detection of orf infection. Journal of Virological Methods 78 : 249-252.
Bora DP, Barman NN, Das SK, Bhanuprakash V, Yogisharadhya R, Venkatesan G Kumar A, Rajbongshi G, Khatoon E, Chakraborty A, Bujarbaruah KM (2012) Identification and phylogenetic analysis of Orf viruses isolated from outbreaks in goats of Assam, a north - eastern state of India. Virus Genes 45: 98-104.
Caravaglio JV , Khachemoune A (2017) Orf Virus Infection in Humans: A Review with a focus on advances in diagnosis and treatment. Journal of Drugs in Dermatology 16: 684-689.
Chandranaik BM, Harish BR, Mallikarjun patil, Byregowda SM, Mamtha GS, Jayakumar SR, Krishnappa G (2005) Outbreak of Orf in sheep: an investigation. Intas Polivet : 66-67.
Delhon G, Tulman ER, Afonso CL (2004) Genomes of the Parapoxviruses: Orf virus and bovine papular stomatitis virus. Journal of Virology 78: 168-177
Fleming SB, Block J, Fraser KM, Mercer AA, Robinson AJ (1993) Conservation of gene structure and arrangement between vaccinia virus and Orf virus. Virology 195: 175-184.
Gelaye E, Achenbach J E , Jenberie S, Gelagay A, Belay A, Loitsch MY, Reingard G, Adama D , Charles EL (2016) Molecular charcetrization of orf virus from sheep and goats in Ethiopia,2008-2013. Virology Journal 13:34.
Hosamani M, Bhanuprakash V, Scagliarini A, Singh RK (2006) Comparative sequence analysis of major envelope protein gene (B2L) of Indian Orf viruses isolated from sheep and goats. Veterinary Microbiology 116: 317-324.
Hosamani M, Yadav S, Kallesh DJ, Mondal B, Bhanuprakash V, Singh RK, (2007) Isolation and characterization of an Indian Orf virus from goats. Zoonoses and Public Health 54: 204-208.
Hosamani M, Scagliarini A., Bhanuprakash V, Mcinnes CJ, Singh RK (2009) Orf: an update on current research and future perspectives. Expert Review of Anti- infective Therapy 7: 879-893.
Housawi FMT, Abu-Elzein E, Gameel A, Mustafa M, Nettleton P (2004) Severe Auzsyk infection in one-month-old camel calves (Camelus dromedarius). Veterinary Archives 74: 467-474
Inoshima Y, Morooka A, Sentsui H (2000) Detection and diagnosis of Parapoxvirus by the PCR. Journal of Virological Methods 84: 201-208.
Inoshima Y, Murakami K, Wu D, Sentsui H (2002) Characterization of parapoxviruses circulating among wild Japanese serows (Capricornis crispus). Microbiology and Immunology 46: 583-587
Kottaridi C, Nomikou K, Teodori l, Savini G, Lelli R, Markoulatos P, Mangana O (2006) Phylogenetic correlation of Greek and Italian Orf virus isolates based on VIR gene. Veterinary Microbiology 16: 310-316.
Kumar AA, Shivachandra SB, Biswas A, Singh VP, Singh VP, Srivastava SK (2004) Prevalent serotypes of Pasteurella multocida isolated from different animal and avian species in India. Veterinary Research Communication 28 : 657-667.
Mckeever DJ, Reid HW, Inglis NF, Herring AJ (1987) A qualitative and quantitative assessment of the humoral antibody response of the sheep to Orf virus infection. Veterinary Microbiology 15: 229-241.
Mohamed, Mahmoud A, Khaled Abdelrahman A, Hatem Soliman (2010) Molecular and virological studies on contagious pustular dermatitis isolates from Egyptian sheep and goats. Research in Veterinary Science 89: 290-294.
OIE (2007) Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees). Office International des Epizooties, Paris, France. Pp739-750.
Saitou N, Nei M (1987) The neighbor joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406-425.
Spyrou V, Valiakos G (2015) Orf virus infection in sheep and goats. Veterinary Microbiology 181: 178-182.
Sullivan JT, Mercer AA, Fleming SB, Robinson AJ (1994) Identification and characterization of an Orf virus homologue of the vaccinia virus gene encoding the major envelope antigen p37K.Virology 202: 968-973
Thompson JD, Higgins DG, Gibson TJ, Clustal W (1994) Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673-4680.