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Abstract


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Volume 7, Issue 3, June Issue - 2019, Pages:335-342


Authors: Sandeep A. Dhenge, Nitin E. Gade, Omprakash Mishra, Mangesh M. Vaidya
Abstract: In present study, caprine Wharton’s jelly derived mesenchymal stem cells (WJ-MSCs) were isolated and characterized by osteogenic and adipogenic differentiation. Total 12 hybrid graphene oxide  nanocomposites (hGO NCs) such  as N2 doped GO-HA NCs, P doped GO-HA NCs, S doped GO-HA NCs, N2 doped GO-SiO2 NCs, P doped GO-SiO2 NCs, S doped GO-SiO2 NCs, N2 doped GO-TiONCs, P doped GO-TiONCs, S doped GO-TiO2 NCs, N2 doped GO-Au NCs, P doped GO-Au NCs and S doped GO-Au NCs were incorporated at doses 100, 50, 25 , 10 and 0 µg/ml in vitrification solution. Caprine WJ-MSCs were cryopreserved by using open pulled straw (OPS) vitrification method and analysed hGO NCs effect on their post thaw viability and culture characteristics. Caprine WJ-MSCs were exhibited normal fibroblastoid morphology and differentiated in to osteogenic and adipogenic lineages. The significant (P<0.01) highest and lowest caprine WJ-MSCs post thaw viability (cumulatively) was observed in P-GO-TiO2 NC and P-GO-HA NC groups, respectively along with all doses (cumulatively) significantly (P<0.01) decreased post thaw cell viability as compared with control. All hGO NCs were significantly (P<0.01) decreased caprine WJ-MSCs post thaw viability at doses 100 and 50 µg/ml as compared to 25, 10 and 0 µg/ml doses. Post thaw caprine WJ-MSCs were grew in normal pattern with similar fibroblast like morphology on days 14. In conclusion, all hGO NCs at doses 50 and 100 µl/ml are cytotoxic and P-GO-TiO2 NC is least decrease caprine WJ-MSCs post thaw viability.
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