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Volume 7, Issue 2, April Issue - 2019, Pages:186-193

Authors: Swapna, K. Tamil Vendan, Mahadevaswamy, D. S. Aswathanarayana, R. C. Gundappagol
Abstract: Seventy five Azotobacter sp were isolated from rhizospheric soils of chilli grown in Raichur, Koppal, Bellary, Yadagiri and Kalaburagi region (parts of Hyderabad Karnataka) and characterized by using both morphological and biochemical tests. Compound Microscopic observation showed that all the isolates were rod shaped and Gram-negative. These isolates were capable of producing mildly heat and stress resistant cyst under nutrient deprivation and physical stress conditions. Interestingly, light brown to dark brown pigmentation varying with isolate are observed after 7 to 14 days after incubation at 37 0C. These isolates were capable of utilizing Protein (gelatin), starch, tryptophan whereas, production of ammonia is observed at the end of protein degradation. Most of the isolates were capable of reducing nitrate, a positive sign for the fixation of free nitrogen. 
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