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Volume 7, Issue 2, April Issue - 2019, Pages:167-177

Authors: Gaurav Singh, Milind Ratnaparkhe, Anil Kumar
Abstract: Transposable elements (jumping genes) are the DNA sequences that jumps (or move) from one genomic region to another. These are found in all living species and are genomically diverse due to their mobility. These are active and can relocate themselves to different locations of the genome. Here, a study has been carried out to identify transposable elements in Glycine max (soybean), Cajanus cajan (pigeon pea) and Phaseolus vulgaris (common bean/green bean/French bean) using previously known elements. In present study homology method of transposable elements identification was used. The identified transposable elements have also been characterized using phylogenetic analysis. Different tools such as Uniprot, BLAST, MSA and PHYLIP were used for this study. A total 12 TEs were taken in G. max, C. cajan and P. vulgaris; these were selected from vicinity of Rpg1b on chromosome 13 of G. max. NCBI BLAST was done for all 12 TEs. Out of 12 TEs 6 were copia, 5 are gypsy and 1 LINE. It is found that copia and gypsy are closely related transposable elements. It is revealed that these TEs have high sequence similarity with the transposons from C. cajan and P. vulgaris indicating that these elements were present before the divergence of these three legumes.
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