Volume 7, Issue 1, February Issue - 2019, Pages:57-64
Authors: Disha N. Patel, Shailesh R. Dave, Vincent J. Braganza, Hasmukh A. Modi
Abstract: The present study deals with bacterial diversity study based on cultivation of the organisms from the Dholera hot springs of Gujarat, India during various seasons. Isolation was carried out by spread plate and streak plate method. Isolates were characterized based on colony and morphological characteristics. Various biochemical tests and 16S rRNA gene analysis were performed for identification of thermophilic bacteria. A total of 18 isolates were cultivated using different types of media. Out of the total 18 isolates, eight were Gram-positive and ten were Gram-negative. Based on the biochemical test of these isolates, 8 were found to produce catalase, 18, 18 and 8 isolates utilized casein, starch, and citrate respectively. Further, 9 isolates were produced H2S by sulphate reduction. Sugar utilization varied in all the isolates. Diversity of the isolates were studied in terms of Simpson, Shanon, Menhinick, Margalef, Berger-Parker and Chao-1 based on the metabolic activity of thermophilic bacteria. Six well-isolated purified colonies were selected for 16S rRNA gene sequence analysis. Based on the molecular identification, these isolated bacterial isolates belonged to genera Bacillus, Brevibacillus, Brevibacterium and Tepidomonas. All these thermophilic isolates may have valuable application at an industrial level and for biotechnological purposes.