Volume 7, Issue 1, February Issue - 2019, Pages:34-41
Authors: R. Madhuri, N. Shivakumar, H. C. Lohithaswa, R. Pavan
Abstract: Hybrid rice is one of the successfully demonstrated technologies, which is feasible and readily adoptable for enhancing the rice production. But availability of stable cytoplasmic male sterility and fertility restoring system is vital for commercial exploitation of heterosis in rice. In the present study, seven testers were identified based on their pollen and spikelet fertility at Hybrid rice section, Mandya which were validated for fertility restoration (Rf) locus by four (RM 1, RM 6100, RM 6344 and RM 1108) reported linked SSR markers before hybridization with 10 CMS lines to develop 70 hybrids. All the four markers showed highly positive association with phenotypic fertility restoration and maintainer type allele. The markers RM 6100, RM 6344 and RM 1108 linked to Rf4 locus located on chromosome 10 and 7. Based on pollen fertility, 23 hybrids were found to be very effective restorers and 47 as partial restorers while, 44 hybrids were found to be very effective restorers and 26 hybrids showed partial restorer behavior based on spikelet fertility. The testers, MSN 71 and MSN 15- 16 behaved as partial restorers for all the CMS lines except CMS 2A. Based on pollen fertility, the lines CMS 5A and CMS 7A had highest number (4) of effective restorers, followed CMS 9A which had three effective restorers. The male parents, MSN 36, KMR 3, PBK 093-1 -4-4-2-1, PBK 095-5-4-5-1 and PBK 091 -3-7-1 -1 were classified as effective restorers for all the CMS lines.