Volume 6, Issue 6, December Issue - 2018, Pages:936-946
Authors: Gulab Khan Rohela, Aftab Ahmad Shabnam, Pawan Shukla, Azra Nahaid Kamili, Mrinal Kanti Ghosh
Abstract: Morus multicaulis cv. Goshoerami is the leading mulberry variety for silkworm rearing under temperate climatic conditions of Jammu and Kashmir, India. However, the propagation of this popular mulberry variety has always remained a point of contention due to its poor rooting response through stem cuttings. It normally takes 4 to 5 years for raising the saplings of this variety through conventional root grafting techniques. Therefore, for quick propagation of this poor rooting popular mulberry variety, a one step in vitro protocol was developed by culturing nodal explants from 2 year old plants on Murashige & Skoog (MS) media supplemented with individual as well as combination of phytohormones. The maximum shoot bud proliferation (6.3± 0.71 in cm) and rooting (14.7± 0.53 in cm) was observed when nodal explants were cultured on the combinational media of BAP (1 mg/L) and IBA (1 mg/L) after 14 days of culture. These in vitro raised plantlets were hardened by using the sterile soil and vermiculite in 2:1 ratio. Only 25 days were required for the micro propagation and hardening of raised plantlets of Goshoerami through this single step protocol. The hardened plantlets were successfully established in the field with 83% survival rate. The developed one step protocol can be used efficiently for the mass propagation of this elite mulberry variety throughout the year with in short span of 25 days.