Abstract: The present study was conducted to determine the alterations in haematological parameters and stress markers in naturally Balantidium coli infected buffaloes, along with this efficacy of polyherbal anti-stressor product Restobal (Restobal®, M/s Ayurvet Limited) was also evaluated against the stress induced by B. coli. In present study, a total of ten apparently healthy buffaloes were selected and considered as control group while twenty buffaloes with natural B. coli infection were included in the disease group and considered for treatment trial. In all the clinical cases, clinical examination and detailed appraisal of haemato-biochemical profiles were carried out. For treatment trial, a total of twenty diseased buffaloes were divided into two groups with ten buffaloes in each group. In therapeutic trials (group I) buffaloes were administered with specific medication against B. coli infection and in herbal trail buffaloes (group II) were administered with specific medication against B. coli infection along with the commercial polyherbal anti stressor product (Restobal). Findings of present study revealed that buffaloes with B. coli infection have reduced haemoglobin levels, total erythrocyte count and monocyte count with increased total leukocyte, neutrophil, lymphocyte, eosinophil count and serum cortisol levels than the apparently healthy buffaloes which indicative of stress due to disease. Buffaloes administered with oral polyherbal anti stressors product showed the significant difference from the group I by elevation of haemoglobin parameters, packed cell volume and serum cortisol levels along with variations in the vital parameters. In conclusion, polyherbal anti stressor product which contains Ocimum sanctum, Phyllanthus emblica, Mangifera indica and Withania somnifera can be recommended along with the specific disease therapeutic protocol in buffaloes to the management of disease induced stress.