Abstract: The present investigation aimed to isolate and characterize endophytic bacteria from six selected medicinal plants (Ocimum sanctum, Aegle marmelos, Cinnamomum cassia, Azadirachta indica, Calotropis procera and Rauwolfia serpentina) with plant growth promoting activities. Total 38 endophytic bacteria were isolated from the studied plant species by following standard microbiological culture methods. All the isolated bacteria were screened for the production of hydrolyzing enzyme and result of study revealed that maximum isolates have positive catalytic activity and this was followed by amylase, lipase and protease activities. While studying the antibacterial activity of the isolates, it was observed that fifteen isolates showed moderate to higher antibacterial activities against Escherichia coli, Pseudomonas aeruginosa & Staphylococcus aureus. Studies on plant growth promoting activity suggested that 8, 12 & 6 isolates were positive for IAA production, siderophore production and phosphate solubilization activity respectively. Further, eight (21.05%) isolates were found positive for ammonia production. Through this investigation, endophytic bacteria isolated from medicinal plants with plant growth promoting activities were reported, that can be further exploited for sustainable agriculture, with detail scientific investigation.