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Volume 6, Issue 4, August Issue - 2018, Pages:739-745

Authors: Chaithra Y, Isloor S, Shivaram B, Suryaprasad V, Sharada R, Rathnamma D, Srinivas K, Veeregowda BM, Narayanaswamy HD, Shome BR, Kundan SP
Abstract: A study was undertaken to evaluate the immune response against Brucella abortus S19 reduced dose vaccine administered through conjunctival route in comparison with B. abortus S19 standard dose administered through subcutaneous (S/C) route. All, 38 Brucella seronegative cattles were grouped as Group 1 with 24 animals (>8 months), Group 2 with 8 female calves (4-8 months), Group 3 with 6 animals (>8 months). On day 0, Group 1, 2 and 3 animals were administered with reduced dose (5-8×109CFU/dose) vaccine through conjunctival route, standard dose (40-80×109CFU/dose) vaccine through S/C route and normal saline through conjunctival route respectively. Blood and serum samples were collected on 0, 21, 60, 90, 120, 150 and 180 Day of post vaccination (DPV). On 120th day, Group1 animals received booster conjunctival vaccine dose (5-8×109CFU/dose). The humoral and cell mediated immune (CMI) response induced by conjunctival and S/C route vaccine was determined by Competitive Enzyme Linked Immuno Sorbant assay (C-ELISA) and Interferon-γ (IFN- γ) assay respectively. The vaccinal antibodies on 21, 60, 90, 120, 150 and 180 DPV were 33.3, 41.6, 17.3, 22.7, 77.2 and 70.1% respectively in conjunctival group and 100, 100, 87.5, 50, 33.3 and 30.2 % respectively in S/C route vaccine group. Stimulation index (>8.89) for IFN-γ response on 21, 60, 90, 120 150 and 180 DPV were 25, 70.8, 52.1, 50, 69.5 and 20% respectively in conjunctival group and 37.5, 37.5, 25, 50, 33.3 and 16.7% respectively in S/C vaccine group. The CMI response was comparatively high on 60 DPV in conjunctival group compared to S/C group animals. Humoral immune response of S/C group animals was 100% on 21 and 60 DPV. After administration of the booster dose vaccinal antibodies increased from 22.7 to 77.2% and stimulation index for IFN-γ response from 50 to 69.5%. Conjunctival route vaccination is safer and easier to dispense than S/C route and it is effective in female cattle >8 months of age. Persistent antibody level is a disadvantage in S/C vaccine which can be overcome by using reduced dose conjunctival route vaccine. If a calf misses S/C vaccination in the age group of 4-8 months an alternate way to immunize animals through conjunctival vaccine can be adopted to achieve maximum vaccination coverage against brucellosis.
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