Abstract: The article presents an overview of the use of different nutrient media during in vitro micropropagation of plants. Further, article also provides information regarding the effect of Driver-Kuniyukinutrient medium on the proliferation and micropropagation of the LC-52 and Gisela-6 clonal rootstock of stone fruit. Result of study revealed that use of the Driver-Kuniyuki nutrient medium have significant effect on micropropagation of LC-52 and Gisela-6 clonal rootstocks of stone fruit and increase the size by 1.15 cm for the LC-52 rootstock and 0.5 cm for the Gizela-6 rootstock per passage.