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Volume 6, Issue 3, June Issue - 2018, Pages:599-605

Authors: Chintan Kapadia, Nidhi Patel, Nitin Patel, Taslim Ahmad
Abstract: Spine gourd is diocious and perennial creeper which can conventionally propagate through seeds, vine cuttings and dividing tubers. The main difficulty in the conventional propagation is associated with the quality and storage of tubers; on the other hand, the micropropagation technique of spine gourd gives true to type plants in large numbers throughout the year. In present study, routine micropropogations method including MS medium for the entire experiment was used. Nodal explants were sterilized by 0.1% mercuric chloride for 10 minutes this was followed by three sterile distilled water wash. Sterile explants were inoculated in full strength MS medium supplemented with different combination and concentration of hormones i.e. 6-Benzyl amino purine (BA), Indole acetic acid (IAA) and Naphthalic acetic acid (NAA) for shoot multiplication. Results of present study revealed that supplementation of NAA (1mg/l) along with BA (1mg/l) induced vigorous and healthy shoots with highest (4.75 ± 0.25) mean no of shoots with average shoot length (cm) of around 5.10 ± 0.04. The regenerated shoots of 4 cm in length were used for rooting purpose. Half strength of MS medium with different concentrations of hormones i.e. Indole butyric acid (IBA), Indole acetic acid (IAA) and Naphthalic acetic acid (NAA) were also used for rooting. The highest rooting percent (86.67 %), number of roots per culture (9.6 ± 0.50) and root length (cm) (4.59 ± 0.09) with lowest percent callusing at cut ends (33.33 %) was reported in the treatment M2 (½ MS + IBA (2.0 mg/l). The well developed shoots with roots were deliberately transferred to the polythene small size glass containing equal mixture of soil and vermicompost. The established plants were finely transplanted in the field conditions.
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