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Volume 6, Issue 2, April Issue - 2018, Pages:324-334

Authors: Nuha M Alhazmi, Khaled M Ghanem, Amani MD El Ahwany, Magda M Aly, Fardos M Bokhari
Abstract: In this work, sequential optimization strategy, based on statistical designs, was employed to enhance the degradation of PHB by Trichoderma asperellum through the measurement of PHB depolymerase specific activity. For PHB degradation screening, two-level Plackett–Burman design was used. Result of study revealed that fermentation medium composition significantly influencing the PHB depolymerase specific activity. Further, it was reported that time of incubation, levels of PHB and concentration of glucose were found to be the major factors influencing the enzyme specific activity. A second three-level Box– Behnken design was applied to acquire the best process conditions for PHB depolymerase specific activity. In this study, the initial basal medium showed a PHB depolymerase specific activity of 40 ng / l / hr / g dry wt and through the two successive statistical design, the final level of PHB depolymerase specific activity was 3230 ng / l / hr / g drywt, this means a nearly of 80 fold increase.
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